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Article

Antiviral Activity of Canine RIG-I against Canine Influenza Virus and Interactions between Canine RIG-I and CIV

by 1,2,3,†, 1,2,3,†, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3, 4, 1,2,3 and 1,2,3,*
1
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
2
Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou 510642, China
3
Guangdong Technological Engineering Research Center for Pet, South China Agricultural University, Guangzhou 510642, China
4
College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510642, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Chao-Nan Lin and Peck Toung Ooi
Viruses 2021, 13(10), 2048; https://doi.org/10.3390/v13102048
Received: 9 September 2021 / Revised: 7 October 2021 / Accepted: 8 October 2021 / Published: 12 October 2021
(This article belongs to the Topic Veterinary Infectious Diseases)
RIG-I functions as a virus sensor that induces a cellular antiviral response. Although it has been investigated in other species, there have been no further studies to date on canine RIG-I against canine influenza virus (CIV). In the present study, we cloned the RIG-I gene of beagle dogs and characterized its expression, subcellular localization, antiviral response, and interactions with CIV proteins. RIG-I was highly expressed and mainly localized in the cytoplasm, with low levels detected in the nucleus. The results revealed that overexpression of the CARD domain of RIG-I and knockdown of RIG-I showed its ability to activate the RLR pathway and induced the expression of downstream interferon-stimulated genes. Moreover, overexpression of canine RIG-I suppressed the replication of CIV. The association between RIG-I and CIV was evaluated with the luciferase assay and by indirect immunofluorescence and bimolecular fluorescence complementation analyses. The results showed that CIV nonstructural protein 1 (NS1) can strongly suppress the RIG-I–mediated innate immune response, and the novel interactions between CIV matrix proteins (M1 and M2) and canine RIG-I were disclosed. These findings provide a basis for investigating the antiviral mechanism of canine RIG-I against CIV, which can lead to effective strategies for preventing CIV infection in dogs. View Full-Text
Keywords: canine RIG-I; antiviral; CIV; interaction canine RIG-I; antiviral; CIV; interaction
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MDPI and ACS Style

Wang, Z.; Ye, S.; Yao, C.; Wang, J.; Mao, J.; Xu, L.; Liu, Y.; Fu, C.; Lu, G.; Li, S. Antiviral Activity of Canine RIG-I against Canine Influenza Virus and Interactions between Canine RIG-I and CIV. Viruses 2021, 13, 2048. https://doi.org/10.3390/v13102048

AMA Style

Wang Z, Ye S, Yao C, Wang J, Mao J, Xu L, Liu Y, Fu C, Lu G, Li S. Antiviral Activity of Canine RIG-I against Canine Influenza Virus and Interactions between Canine RIG-I and CIV. Viruses. 2021; 13(10):2048. https://doi.org/10.3390/v13102048

Chicago/Turabian Style

Wang, Zhen, Shaotang Ye, Congwen Yao, Ji Wang, Jianwei Mao, Liang Xu, Yongbo Liu, Cheng Fu, Gang Lu, and Shoujun Li. 2021. "Antiviral Activity of Canine RIG-I against Canine Influenza Virus and Interactions between Canine RIG-I and CIV" Viruses 13, no. 10: 2048. https://doi.org/10.3390/v13102048

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