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Volume 13
Issue 10
10.3390/v13102048
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Article

Antiviral Activity of Canine RIG-I against Canine Influenza Virus and Interactions between Canine RIG-I and CIV

by
Zhen Wang
1,2,3,†,
Shaotang Ye
1,2,3,†,
Congwen Yao
1,2,3,
Ji Wang
1,2,3,
Jianwei Mao
1,2,3,
Liang Xu
1,2,3,
Yongbo Liu
1,2,3,
Cheng Fu
4,
Gang Lu
1,2,3 and
Shoujun Li
1,2,3,*
1
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
2
Guangdong Provincial Key Laboratory of Prevention and Control for Severe Clinical Animal Diseases, Guangzhou 510642, China
3
Guangdong Technological Engineering Research Center for Pet, South China Agricultural University, Guangzhou 510642, China
4
College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510642, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editors: Chao-Nan Lin and Peck Toung Ooi
Viruses 2021, 13(10), 2048; https://doi.org/10.3390/v13102048
Received: 9 September 2021 / Revised: 7 October 2021 / Accepted: 8 October 2021 / Published: 12 October 2021
(This article belongs to the Topic Veterinary Infectious Diseases)
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Abstract

RIG-I functions as a virus sensor that induces a cellular antiviral response. Although it has been investigated in other species, there have been no further studies to date on canine RIG-I against canine influenza virus (CIV). In the present study, we cloned the RIG-I gene of beagle dogs and characterized its expression, subcellular localization, antiviral response, and interactions with CIV proteins. RIG-I was highly expressed and mainly localized in the cytoplasm, with low levels detected in the nucleus. The results revealed that overexpression of the CARD domain of RIG-I and knockdown of RIG-I showed its ability to activate the RLR pathway and induced the expression of downstream interferon-stimulated genes. Moreover, overexpression of canine RIG-I suppressed the replication of CIV. The association between RIG-I and CIV was evaluated with the luciferase assay and by indirect immunofluorescence and bimolecular fluorescence complementation analyses. The results showed that CIV nonstructural protein 1 (NS1) can strongly suppress the RIG-I–mediated innate immune response, and the novel interactions between CIV matrix proteins (M1 and M2) and canine RIG-I were disclosed. These findings provide a basis for investigating the antiviral mechanism of canine RIG-I against CIV, which can lead to effective strategies for preventing CIV infection in dogs. View Full-Text
Keywords: canine RIG-I; antiviral; CIV; interaction canine RIG-I; antiviral; CIV; interaction
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MDPI and ACS Style

Wang, Z.; Ye, S.; Yao, C.; Wang, J.; Mao, J.; Xu, L.; Liu, Y.; Fu, C.; Lu, G.; Li, S. Antiviral Activity of Canine RIG-I against Canine Influenza Virus and Interactions between Canine RIG-I and CIV. Viruses 2021, 13, 2048. https://doi.org/10.3390/v13102048

AMA Style

Wang Z, Ye S, Yao C, Wang J, Mao J, Xu L, Liu Y, Fu C, Lu G, Li S. Antiviral Activity of Canine RIG-I against Canine Influenza Virus and Interactions between Canine RIG-I and CIV. Viruses. 2021; 13(10):2048. https://doi.org/10.3390/v13102048

Chicago/Turabian Style

Wang, Zhen, Shaotang Ye, Congwen Yao, Ji Wang, Jianwei Mao, Liang Xu, Yongbo Liu, Cheng Fu, Gang Lu, and Shoujun Li. 2021. "Antiviral Activity of Canine RIG-I against Canine Influenza Virus and Interactions between Canine RIG-I and CIV" Viruses 13, no. 10: 2048. https://doi.org/10.3390/v13102048

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