Genetic and Antigenetic Characterization of the Novel Kotalahti Bat Lyssavirus (KBLV)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Rabies Tissue Culture Isolation Test (RTCIT)
2.2. In Vivo Studies
2.3. Next Generation Sequencing (NGS) and Phylogenetic Analysis
2.4. Plasmids and cDNA Cloning
2.5. G-Deleted Virus and Generation of Pseudotyped RABV
2.6. Cross-Neutralization of KBLV by Human Sera
3. Results
3.1. Virus Isolation from Tissue Samples Failed
3.2. NGS, Sequence Analysis and Phylogeny
3.3. Expression of KBLV Proteins in Transfected Cells
3.4. Cross-Neutralization
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Calvelage, S.; Tammiranta, N.; Nokireki, T.; Gadd, T.; Eggerbauer, E.; Zaeck, L.M.; Potratz, M.; Wylezich, C.; Höper, D.; Müller, T.; et al. Genetic and Antigenetic Characterization of the Novel Kotalahti Bat Lyssavirus (KBLV). Viruses 2021, 13, 69. https://doi.org/10.3390/v13010069
Calvelage S, Tammiranta N, Nokireki T, Gadd T, Eggerbauer E, Zaeck LM, Potratz M, Wylezich C, Höper D, Müller T, et al. Genetic and Antigenetic Characterization of the Novel Kotalahti Bat Lyssavirus (KBLV). Viruses. 2021; 13(1):69. https://doi.org/10.3390/v13010069
Chicago/Turabian StyleCalvelage, Sten, Niina Tammiranta, Tiina Nokireki, Tuija Gadd, Elisa Eggerbauer, Luca M. Zaeck, Madlin Potratz, Claudia Wylezich, Dirk Höper, Thomas Müller, and et al. 2021. "Genetic and Antigenetic Characterization of the Novel Kotalahti Bat Lyssavirus (KBLV)" Viruses 13, no. 1: 69. https://doi.org/10.3390/v13010069
APA StyleCalvelage, S., Tammiranta, N., Nokireki, T., Gadd, T., Eggerbauer, E., Zaeck, L. M., Potratz, M., Wylezich, C., Höper, D., Müller, T., Finke, S., & Freuling, C. M. (2021). Genetic and Antigenetic Characterization of the Novel Kotalahti Bat Lyssavirus (KBLV). Viruses, 13(1), 69. https://doi.org/10.3390/v13010069