The Genetic Diversification of a Single Bluetongue Virus Strain Using an In Vitro Model of Alternating-Host Transmission
Abstract
:1. Introduction
2. Materials and Methods
2.1. Virus Isolation
2.2. Cells
2.3. Virus Infections
2.4. RT-PCR
2.5. WGS Library Preparation
2.6. Bioinformatics
2.7. Population Genetics
2.8. Statistics
3. Results
3.1. Whole Genome Sequencing and Detection of Single Nucleotide Consensus Changes
3.2. Measures of Genetic Diversity across Passages and Cell Types
3.3. Measures of Genetic Divergence across Passages and Cell Types
3.4. Measures of Selection across Passages and Cell Types
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Kopanke, J.H.; Lee, J.S.; Stenglein, M.D.; Mayo, C.E. The Genetic Diversification of a Single Bluetongue Virus Strain Using an In Vitro Model of Alternating-Host Transmission. Viruses 2020, 12, 1038. https://doi.org/10.3390/v12091038
Kopanke JH, Lee JS, Stenglein MD, Mayo CE. The Genetic Diversification of a Single Bluetongue Virus Strain Using an In Vitro Model of Alternating-Host Transmission. Viruses. 2020; 12(9):1038. https://doi.org/10.3390/v12091038
Chicago/Turabian StyleKopanke, Jennifer H., Justin S. Lee, Mark D. Stenglein, and Christie E. Mayo. 2020. "The Genetic Diversification of a Single Bluetongue Virus Strain Using an In Vitro Model of Alternating-Host Transmission" Viruses 12, no. 9: 1038. https://doi.org/10.3390/v12091038