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Article

Minimum Infective Dose of a Lumpy Skin Disease Virus Field Strain from North Macedonia

1
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, D-17493 Greifswald-Insel Riems, Germany
2
Faculty of Veterinary Medicine, University “Ss. Cyril and Methodius” Skopje, Lazar Pop Trajkov 5–7, 1000 Skopje, North Macedonia
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(7), 768; https://doi.org/10.3390/v12070768
Received: 12 June 2020 / Revised: 6 July 2020 / Accepted: 13 July 2020 / Published: 16 July 2020
(This article belongs to the Section Animal Viruses)
Infection with Lumpy Skin Disease virus (LSDV), as well as infections with other Capripox virus species, are described as the most severe pox diseases of production animals and are therefore listed as notifiable diseases under the guidelines of the World Organization for Animal Health (OIE). To our knowledge there is only a single study examining dose dependency, clinical course, viremia, virus shedding, as well as serological response following experimental LSDV “Neethling” inoculation. Here, we inoculated cattle with four different doses of LSDV strain “Macedonia2016”, a recently characterized virulent LSDV field strain, and examined clinical symptoms, viremia, viral shedding, and seroconversion. Interestingly, around 400 cell culture infectious dose50 (CCID50) of LSDV-“Macedonia2016” were sufficient to induce generalized Lumpy Skin Disease (LSD) in two out of six cattle but with a different incubation time, whereas the other animals of this group showed only a mild course of LSD. However, differences in incubation time, viral loads, serology, and in the clinical scoring could not be observed in the other three groups. In summary, we concluded that experimental LSDV infection of cattle with an infectious virus titer of 105 to 106 CCID50/mL of “Macedonia2016” provides a robust and sufficient challenge model for future studies. View Full-Text
Keywords: Capripox; Lumpy Skin Disease; LSDV; minimum infective dose; experimental inoculation; field strain; challenge model Capripox; Lumpy Skin Disease; LSDV; minimum infective dose; experimental inoculation; field strain; challenge model
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MDPI and ACS Style

Wolff, J.; Krstevski, K.; Beer, M.; Hoffmann, B. Minimum Infective Dose of a Lumpy Skin Disease Virus Field Strain from North Macedonia. Viruses 2020, 12, 768. https://doi.org/10.3390/v12070768

AMA Style

Wolff J, Krstevski K, Beer M, Hoffmann B. Minimum Infective Dose of a Lumpy Skin Disease Virus Field Strain from North Macedonia. Viruses. 2020; 12(7):768. https://doi.org/10.3390/v12070768

Chicago/Turabian Style

Wolff, Janika, Kiril Krstevski, Martin Beer, and Bernd Hoffmann. 2020. "Minimum Infective Dose of a Lumpy Skin Disease Virus Field Strain from North Macedonia" Viruses 12, no. 7: 768. https://doi.org/10.3390/v12070768

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