Pteropine Orthoreovirus in an Angolan Soft-Furred Fruit Bat (Lissonycteris angolensis) in Uganda Dramatically Expands the Global Distribution of an Emerging Bat-Borne Respiratory Virus
Abstract
1. Introduction
2. Materials and Methods
2.1. Collection of Samples
2.2. Metagenomics
2.3. Phylogenetics of PRV16K Segments and Asia Pacific PRVs
2.4. Detection of Positive Selection on Cell-Attachment Protein (σC)
2.5. Correlation of Geographic Distance with Patristic Distance of Segments S1-4
3. Results
3.1. Sequencing and PRV Genome Assembly
3.2. Evolutionary Relationships among PRV Segments
3.3. Geographic Relationships among PRV Segments S1-4
3.4. Diversifying Selection on Cell-Attachment Protein (σC)
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Isolate | Common Name | Year | Host | Country of Origin |
---|---|---|---|---|
PRV1NB | Nelson Bay virus | 1968 | Bat (Pteropus policephalus) | Australia |
PRV2P | Pulau virus | 1999 | Bat (Pteropus hypomelanus) | Malaysia |
PRV3M | Melaka virus | 2006 | Human | Malaysia |
PRV4K | Kampar virus | 2006 | Human | Malaysia |
PRV5HK | HK23629/07 | 2007 | Human | Indonesia |
PRV6XR | Xi River virus | 2006 | Bat (Rousettus leschenaultii) | China |
PRV7S | Sikamat virus | 2010 | Human | Malaysia |
PRV8B | HK46886/09 | 2009 | Human | Indonesia |
PRV9HK | HK50842/10 | 2010 | Human | Indonesia |
PRV10M | Miyazaki-Bali 2007 | 2007 | Human | Indonesia |
PRV11C | Cangyuan virus | 2012 | Bat (Rousettus leschenaultii) | China |
PRV12I | Indonesia/2010 | 2010 | Bat (Pteropus vampyrus) | Indonesia |
PRV13P | Samal-24 | 2013 | Bat (Eonycteris spelaea) | Philippines |
PRV14P | Talikud-80 | 2013 | Bat (Rousettus amplexicaudatus) | Philippines |
PRV15G | Garut-69 | 2017 | Bat (Pteropus vampyrus) | Indonesia |
PRV16K* | Kasama virus | 2017 | Bat (Lissonycteris angolensis ruwenzorii) | Uganda |
PRV Genome Segment | Virus Protein(s) | Mantel’s r (rM) | p-Value (Two-Tailed) [99% CI] |
---|---|---|---|
S1 | P10 (FAST), P17 (unknown), σC (cell attachment protein) | 0.462 | <0.0001 * |
S2 | σ1 (major inner-capsid protein) | 0.229 | 0.031 [0.027–0.036] * |
S3 | σNS (nonstructural replication protein) | 0.132 | 0.179 [0.169–0.189] |
S4 | σ2 (major outer-capsid protein) | 0.373 | <0.0001 * |
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Bennett, A.J.; Goldberg, T.L. Pteropine Orthoreovirus in an Angolan Soft-Furred Fruit Bat (Lissonycteris angolensis) in Uganda Dramatically Expands the Global Distribution of an Emerging Bat-Borne Respiratory Virus. Viruses 2020, 12, 740. https://doi.org/10.3390/v12070740
Bennett AJ, Goldberg TL. Pteropine Orthoreovirus in an Angolan Soft-Furred Fruit Bat (Lissonycteris angolensis) in Uganda Dramatically Expands the Global Distribution of an Emerging Bat-Borne Respiratory Virus. Viruses. 2020; 12(7):740. https://doi.org/10.3390/v12070740
Chicago/Turabian StyleBennett, Andrew J., and Tony L. Goldberg. 2020. "Pteropine Orthoreovirus in an Angolan Soft-Furred Fruit Bat (Lissonycteris angolensis) in Uganda Dramatically Expands the Global Distribution of an Emerging Bat-Borne Respiratory Virus" Viruses 12, no. 7: 740. https://doi.org/10.3390/v12070740
APA StyleBennett, A. J., & Goldberg, T. L. (2020). Pteropine Orthoreovirus in an Angolan Soft-Furred Fruit Bat (Lissonycteris angolensis) in Uganda Dramatically Expands the Global Distribution of an Emerging Bat-Borne Respiratory Virus. Viruses, 12(7), 740. https://doi.org/10.3390/v12070740