Cathepsin B Protease Facilitates Chikungunya Virus Envelope Protein-Mediated Infection Via Endocytosis or Macropinocytosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells
2.2. Plasmids
2.3. Pseudotyped MLV Vector
2.4. Cell Viability
2.5. Macropinocytosis Assay
2.6. Mouse Antiserum against CHIKV E2 Protein
2.7. Western Immunoblotting
2.8. RT-PCR
2.9. Statistical Analysis
3. Results
3.1. Characterization of CHIKV-Pseudotyped Retrovirus Vector
3.2. Endocytosis Is Required for CHIKV-Pseudotyped MLV Vector Infection in 293T but Not in TE671 Cells
3.3. Macropinocytosis Is Required for CHIKV-Pseudotyped MLV Vector Infection in TE671 Cells but Not in 293T Cells
3.4. Cathepsin Protease Is Required for CHIKV-Pseudotyped MLV Vector Infection in 293T and TE671 Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Izumida, M.; Hayashi, H.; Tanaka, A.; Kubo, Y. Cathepsin B Protease Facilitates Chikungunya Virus Envelope Protein-Mediated Infection Via Endocytosis or Macropinocytosis. Viruses 2020, 12, 722. https://doi.org/10.3390/v12070722
Izumida M, Hayashi H, Tanaka A, Kubo Y. Cathepsin B Protease Facilitates Chikungunya Virus Envelope Protein-Mediated Infection Via Endocytosis or Macropinocytosis. Viruses. 2020; 12(7):722. https://doi.org/10.3390/v12070722
Chicago/Turabian StyleIzumida, Mai, Hideki Hayashi, Atsushi Tanaka, and Yoshinao Kubo. 2020. "Cathepsin B Protease Facilitates Chikungunya Virus Envelope Protein-Mediated Infection Via Endocytosis or Macropinocytosis" Viruses 12, no. 7: 722. https://doi.org/10.3390/v12070722