Next Article in Journal
The Single-Stranded RNA Bacteriophage Qβ Adapts Rapidly to High Temperatures: An Evolution Experiment
Next Article in Special Issue
Special Issue “Viral Evasion or Suppression of Host Immunity”
Previous Article in Journal
Innate Immune Components That Regulate the Pathogenesis and Resolution of hRSV and hMPV Infections
Previous Article in Special Issue
The Viral Macrodomain Counters Host Antiviral ADP-Ribosylation
Article

n-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8+ T Cells

1
Department of Life Science, Chung-Ang University, Seoul 06974, Korea
2
Department of Chemistry, Chung-Ang University, Seoul 06974, Korea
3
Division of Biotechnology, College of Environmental and Bioresources, Jeonbuk National University, Iksan 54596, Korea
4
Department of Biotechnology, the Catholic University of Korea, Bucheon 14662, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2020, 12(6), 639; https://doi.org/10.3390/v12060639
Received: 11 May 2020 / Revised: 9 June 2020 / Accepted: 10 June 2020 / Published: 12 June 2020
(This article belongs to the Special Issue Viral Evasion or Suppression of Host Immunity)
The immune-suppressive effects of omega-3 (n-3) polyunsaturated fatty acids (PUFAs) on T cells have been observed via multiple in vitro and in vivo models. However, the precise mechanism that causes these effects is still undefined. In this study, we investigated whether n-3 PUFAs regulated T cell receptor (TCR) and peptide-major histocompatibility complex (pMHC) interactions. The expansion of anti-viral CD8+ T cells that endogenously synthesize n-3 PUFAs (FAT-1) dramatically decreased upon lymphocytic choriomeningitis virus (LCMV) infection in vivo. This decrease was not caused by the considerable reduction of TCR expression or the impaired chemotactic activity of T cells. Interestingly, a highly inclined and laminated optical sheet (HILO) microscopic analysis revealed that the TCR motility was notably reduced on the surface of the FAT-1 CD8+ T cells compared to the wild type (WT) CD8+ T cells. Importantly, the adhesion strength of the FAT-1 CD8+ T cells to the peptide-MHC was significantly lower than that of the WT CD8+T cells. Consistent with this result, treatment with docosahexaenoic acid (DHA), one type of n-3 PUFA, significantly decreased CD8+ T cell adhesion to the pMHC. Collectively, our results reveal a novel mechanism through which n-3 PUFAs decrease TCR-pMHC interactions by modulating TCR mobility on CD8+ T cell surfaces. View Full-Text
Keywords: omega-3; CD8+ T cells; LCMV; TCR-pMHC interaction omega-3; CD8+ T cells; LCMV; TCR-pMHC interaction
Show Figures

Figure 1

MDPI and ACS Style

Lim, Y.; Kim, S.; Kim, S.; Kim, D.-I.; Kang, K.W.; Hong, S.-H.; Lee, S.-M.; Koh, H.R.; Seo, Y.-J. n-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8+ T Cells. Viruses 2020, 12, 639. https://doi.org/10.3390/v12060639

AMA Style

Lim Y, Kim S, Kim S, Kim D-I, Kang KW, Hong S-H, Lee S-M, Koh HR, Seo Y-J. n-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8+ T Cells. Viruses. 2020; 12(6):639. https://doi.org/10.3390/v12060639

Chicago/Turabian Style

Lim, Younghyun; Kim, Seyoung; Kim, Sehoon; Kim, Dong-In; Kang, Kyung W.; Hong, So-Hee; Lee, Sang-Myeong; Koh, Hye R.; Seo, Young-Jin. 2020. "n-3 Polyunsaturated Fatty Acids Impede the TCR Mobility and the TCR–pMHC Interaction of Anti-Viral CD8+ T Cells" Viruses 12, no. 6: 639. https://doi.org/10.3390/v12060639

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop