Reduced-Beclin1-Expressing Mice Infected with Zika-R103451 and Viral-Associated Pathology during Pregnancy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Viral Propagation
2.2. Ethics Statement
2.3. Animal Model and Timed Pregnancy
2.4. Real Time PCR
2.5. Primer Sequences
2.6. Hematoxylin & Eosin (H&E)
2.7. Murine Mixed Glial Cell Culture
2.8. ELISA
2.9. Plaque Assay
2.10. Immunohistochemistry
2.11. Western Blotting
2.12. Statistical Analysis
3. Results
3.1. Transiently Immunosuppressed Pregnant Becn1+/+ and Becn1+/− Dams Are Susceptible to ZIKV Infection
3.2. Growth Impairment in Pups Exposed to ZIKV In Utero
3.3. Potential Factors Mediating the Pathology in Pups Exposed to ZIKV In Utero
3.4. Reduced Beclin1 Exacerbates Secretion of Inflammatory Molecules in ZIKV-Infected Glia In Vitro
3.5. ZIKV- Proteins NS1 and E Causes Increase in the Secretion of Inflammatory Molecules
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Karuppan, M.K.M.; Ojha, C.R.; Rodriguez, M.; Lapierre, J.; Aman, M.J.; Kashanchi, F.; Toborek, M.; Nair, M.; El-Hage, N. Reduced-Beclin1-Expressing Mice Infected with Zika-R103451 and Viral-Associated Pathology during Pregnancy. Viruses 2020, 12, 608. https://doi.org/10.3390/v12060608
Karuppan MKM, Ojha CR, Rodriguez M, Lapierre J, Aman MJ, Kashanchi F, Toborek M, Nair M, El-Hage N. Reduced-Beclin1-Expressing Mice Infected with Zika-R103451 and Viral-Associated Pathology during Pregnancy. Viruses. 2020; 12(6):608. https://doi.org/10.3390/v12060608
Chicago/Turabian StyleKaruppan, Mohan Kumar Muthu, Chet Raj Ojha, Myosotys Rodriguez, Jessica Lapierre, M. Javad Aman, Fatah Kashanchi, Michal Toborek, Madhavan Nair, and Nazira El-Hage. 2020. "Reduced-Beclin1-Expressing Mice Infected with Zika-R103451 and Viral-Associated Pathology during Pregnancy" Viruses 12, no. 6: 608. https://doi.org/10.3390/v12060608