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Article

Identification and Functional Analysis of Temperate Siphoviridae Bacteriophages of Acinetobacter baumannii

1
Department of Bacteriology and Immunology, Medicum, Human Microbiome Research Program, Faculty of Medicine, University of Helsinki, 00014 UH Helsinki, Finland
2
Department of Botany and Microbiology, Faculty of Science, Damietta University, 34511 New Damietta, Egypt
3
Division of Clinical Microbiology, Helsinki University Hospital, HUSLAB, 00290 Helsinki, Finland
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(6), 604; https://doi.org/10.3390/v12060604
Received: 17 April 2020 / Revised: 25 May 2020 / Accepted: 29 May 2020 / Published: 31 May 2020
(This article belongs to the Section Bacterial Viruses)
Acinetobacter baumannii is an opportunistic pathogen that presents a serious clinical challenge due to its increasing resistance to all available antibiotics. Phage therapy has been introduced recently to treat antibiotic-incurable A. baumannii infections. In search for new A. baumannii specific bacteriophages, 20 clinical A. baumannii strains were used in two pools in an attempt to enrich phages from sewage. The enrichment resulted in induction of resident prophage(s) and three temperate bacteriophages, named vB_AbaS_fEg-Aba01, vB_AbaS_fLi-Aba02 and vB_AbaS_fLi-Aba03, all able to infect only one strain (#6597) of the 20 clinical strains, were isolated. Morphological characteristics obtained by transmission electron microscopy together with the genomic information revealed that the phages belong to the family Siphoviridae. The ca. 35 kb genomic sequences of the phages were >99% identical to each other. The linear ds DNA genomes of the phages contained 10 nt cohesive end termini, 52–54 predicted genes, an attP site and one tRNA gene each. A database search revealed an >99% identical prophage in the genome of A. baumannii strain AbPK1 (acc. no. CP024576.1). Over 99% identical prophages were also identified from two of the original 20 clinical strains (#5707 and #5920) and both were shown to be spontaneously inducible, thus very likely being the origins of the isolated phages. The phage vB_AbaS_fEg-Aba01 was also able to lysogenize the susceptible strain #6597 demonstrating that it was fully functional. The phages showed a very narrow host range infecting only two A. baumannii strains. In conclusion, we have isolated and characterized three novel temperate Siphoviridae phages that infect A. baumannii. View Full-Text
Keywords: Acinetobacter baumannii; temperate; bacteriophage; Siphoviridae; genome; att site; prophage; cohesive ends Acinetobacter baumannii; temperate; bacteriophage; Siphoviridae; genome; att site; prophage; cohesive ends
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MDPI and ACS Style

Badawy, S.; Pajunen, M.I.; Haiko, J.; Baka, Z.A.M.; Abou-Dobara, M.I.; El-Sayed, A.K.A.; Skurnik, M. Identification and Functional Analysis of Temperate Siphoviridae Bacteriophages of Acinetobacter baumannii. Viruses 2020, 12, 604. https://doi.org/10.3390/v12060604

AMA Style

Badawy S, Pajunen MI, Haiko J, Baka ZAM, Abou-Dobara MI, El-Sayed AKA, Skurnik M. Identification and Functional Analysis of Temperate Siphoviridae Bacteriophages of Acinetobacter baumannii. Viruses. 2020; 12(6):604. https://doi.org/10.3390/v12060604

Chicago/Turabian Style

Badawy, Shimaa, Maria I. Pajunen, Johanna Haiko, Zakaria A.M. Baka, Mohamed I. Abou-Dobara, Ahmed K.A. El-Sayed, and Mikael Skurnik. 2020. "Identification and Functional Analysis of Temperate Siphoviridae Bacteriophages of Acinetobacter baumannii" Viruses 12, no. 6: 604. https://doi.org/10.3390/v12060604

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