Indigenous versus Lessepsian Hosts: Nervous Necrosis Virus (NNV) in Eastern Mediterranean Sea Fish
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fish and Tissue Sampling
2.2. RNA extraction and cDNA synthesis by RT-PCR
2.3. RNA1 and RNA2 PCR amplification
2.4. Sequencing and Phylogenetic Analysis
2.5. Statistical Analysis
3. Results
4. Discussion and Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Host Species/Isolate | Origin | NNV Strain | RNA1 | RNA2 |
---|---|---|---|---|
Epinephelus akaara | China | RGNNV | - | AY744705 |
G9508KS | n.a | RGNNV | - | AY690596 |
Dicentrarchus labrax | Tunisia | RGNNV | FJ789783 | - |
Sardina pilchardus | Italy, wild | RGNNV | JN189868 | JN190021 |
Epinephelus spp. | Greece, wild | RGNNV | JN189823 | JN189975 |
Mullus barbatus | Croatia, wild | RGNNV | JN189808 | JN189962 |
Epinephelus aeneus | Israel (Med. Sea b), wild | RGNNV | - | AY284965 |
Lates calcarifer | Israel (Red Sea), farmed | RGNNV | - | AY284973 |
Sparus aurata | Portugal, farmed | RG/SJ a | JN189844 | JN189916 |
Pseudocaranx dentex | Japan | SJNNV | AB056571 | AB056572 |
Verasper moseri | Japan | BFNNV | EU826137 | D38635 |
TPKag93 | Japan | TPNNV | EU236148 | EU236149 |
2016 | 2017 | Total | |||||||
---|---|---|---|---|---|---|---|---|---|
Fish Species | n | Positive | % Prevalence | n | Positive | % Prevalence | n | Positive | % Prevalence |
Lithognathus mormyrus | 30 | 4 | 13.33 | 20 | 3 | 15 | 50 | 7 | 14 |
Sardinella aurita | 30 | 0 | 0 | 28 | 3 | 10.71 | 58 | 3 | 5.17 |
Nemipterus randalli * | 29 | 15 | 51.72 | 31 | 16 | 51.61 | 60 | 31 | 51.67 |
Saurida lessepsianus * | 38 | 11 | 28.95 | 30 | 3 | 10 | 68 | 14 | 20.59 |
Total | 127 | 30 | 23.62 | 109 | 25 | 22.94 | 236 | 55 | 23.31 |
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Lampert, Y.; Berzak, R.; Davidovich, N.; Diamant, A.; Stern, N.; Scheinin, A.P.; Tchernov, D.; Morick, D. Indigenous versus Lessepsian Hosts: Nervous Necrosis Virus (NNV) in Eastern Mediterranean Sea Fish. Viruses 2020, 12, 430. https://doi.org/10.3390/v12040430
Lampert Y, Berzak R, Davidovich N, Diamant A, Stern N, Scheinin AP, Tchernov D, Morick D. Indigenous versus Lessepsian Hosts: Nervous Necrosis Virus (NNV) in Eastern Mediterranean Sea Fish. Viruses. 2020; 12(4):430. https://doi.org/10.3390/v12040430
Chicago/Turabian StyleLampert, Yael, Ran Berzak, Nadav Davidovich, Arik Diamant, Nir Stern, Aviad P. Scheinin, Dan Tchernov, and Danny Morick. 2020. "Indigenous versus Lessepsian Hosts: Nervous Necrosis Virus (NNV) in Eastern Mediterranean Sea Fish" Viruses 12, no. 4: 430. https://doi.org/10.3390/v12040430