Swine Host Protein Coiled-Coil Domain-Containing 115 (CCDC115) Interacts with Classical Swine Fever Virus Structural Glycoprotein E2 during Virus Replication
Abstract
:1. Introduction
2. Materials and Methods
2.1. Viruses and Cells
2.2. Proximity Ligation Assay
2.3. Yeast Two-Hybrid Screening for Disruption of CCDC115 Binding E2
2.4. Construction of CSFV E2ΔCCDC115v Mutant
2.5. Ethics Statement
2.6. Animal Infections
3. Results
3.1. CSFV E2 and Swine Host Protein CCDC115 Interact in CSFV-Infected Cells
3.2. Identification of CSFV E2 Residues Critical for CCDC115 Interaction
3.3. Development of CSFV E2ΔCCDC115v Mutant
3.4. Assessment of Mutant Virus E2ΔCCDC115v Replication In Vitro
3.5. Assessment of E2ΔCCDC115v in CSFV Virulence in Swine
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Fever | |||||
---|---|---|---|---|---|
Treatment (1) | No. of Survivors/Total | Mean Time to Death (Days ± SD) | No. of Days to Onset (Days ± SD | Duration; No. of Days (Days ± SD) | Maximum Daily Temp (°C ± SD) |
BICv | 0/5 | 7 (0) | 5.2 (0.84) | 1.8 (0.84) | 40.01 (0.17) |
CSFV E2ΔCCDC115v | 0/5 | (0.89) | 8.6 (0.89) | 1.6 (0.89) | 40.62 (0.18) |
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Vuono, E.A.; Ramirez-Medina, E.; Berggren, K.; Rai, A.; Pruitt, S.; Silva, E.; Velazquez-Salinas, L.; Gladue, D.P.; Borca, M.V. Swine Host Protein Coiled-Coil Domain-Containing 115 (CCDC115) Interacts with Classical Swine Fever Virus Structural Glycoprotein E2 during Virus Replication. Viruses 2020, 12, 388. https://doi.org/10.3390/v12040388
Vuono EA, Ramirez-Medina E, Berggren K, Rai A, Pruitt S, Silva E, Velazquez-Salinas L, Gladue DP, Borca MV. Swine Host Protein Coiled-Coil Domain-Containing 115 (CCDC115) Interacts with Classical Swine Fever Virus Structural Glycoprotein E2 during Virus Replication. Viruses. 2020; 12(4):388. https://doi.org/10.3390/v12040388
Chicago/Turabian StyleVuono, Elizabeth A., Elizabeth Ramirez-Medina, Keith Berggren, Ayushi Rai, Sarah Pruitt, Ediane Silva, Lauro Velazquez-Salinas, Douglas P. Gladue, and Manuel V. Borca. 2020. "Swine Host Protein Coiled-Coil Domain-Containing 115 (CCDC115) Interacts with Classical Swine Fever Virus Structural Glycoprotein E2 during Virus Replication" Viruses 12, no. 4: 388. https://doi.org/10.3390/v12040388