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Current Trends in Diagnostics of Viral Infections of Unknown Etiology

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FSBI “Center of Strategic Planning” of the Ministry of Health, 119435 Moscow, Russia
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I.M. Sechenov First Moscow State Medical University, 119146 Moscow, Russia
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Moscow Institute of Physics and Technology, National Research University, 117303 Moscow, Russia
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Pasteur Institute, Federal Service on Consumers’ Rights Protection and Human Well-Being Surveillance, 197101 Saint-Petersburg, Russia
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Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov First Moscow State Medical University, 119146 Moscow, Russia
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Author to whom correspondence should be addressed.
Viruses 2020, 12(2), 211; https://doi.org/10.3390/v12020211
Received: 16 December 2019 / Revised: 10 February 2020 / Accepted: 12 February 2020 / Published: 14 February 2020
(This article belongs to the Section Animal Viruses)
Viruses are evolving at an alarming rate, spreading and inconspicuously adapting to cutting-edge therapies. Therefore, the search for rapid, informative and reliable diagnostic methods is becoming urgent as ever. Conventional clinical tests (PCR, serology, etc.) are being continually optimized, yet provide very limited data. Could high throughput sequencing (HTS) become the future gold standard in molecular diagnostics of viral infections? Compared to conventional clinical tests, HTS is universal and more precise at profiling pathogens. Nevertheless, it has not yet been widely accepted as a diagnostic tool, owing primarily to its high cost and the complexity of sample preparation and data analysis. Those obstacles must be tackled to integrate HTS into daily clinical practice. For this, three objectives are to be achieved: (1) designing and assessing universal protocols for library preparation, (2) assembling purpose-specific pipelines, and (3) building computational infrastructure to suit the needs and financial abilities of modern healthcare centers. Data harvested with HTS could not only augment diagnostics and help to choose the correct therapy, but also facilitate research in epidemiology, genetics and virology. This information, in turn, could significantly aid clinicians in battling viral infections. View Full-Text
Keywords: viruses; viral infections; diagnostics; sequencing; PCR; NGS; HTS; single-molecule sequencing; bioinformatics viruses; viral infections; diagnostics; sequencing; PCR; NGS; HTS; single-molecule sequencing; bioinformatics
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Kiselev, D.; Matsvay, A.; Abramov, I.; Dedkov, V.; Shipulin, G.; Khafizov, K. Current Trends in Diagnostics of Viral Infections of Unknown Etiology. Viruses 2020, 12, 211.

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