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In Vitro Evaluation of a Phage Cocktail Controlling Infections with Escherichia coli

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Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany
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Institute for Food Quality and Food Safety, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany
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FINK TEC GmbH, Oberster Kamp 23, 59069 Hamm, Germany
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Food Inspection, Animal Welfare and Veterinary Service of the Land of Bremen, Border Control Post Bremerhaven, Senator-Borttscheller-Straße 8, 27568 Bremerhaven, Germany
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Central Facility for Microscopy, Helmholtz-Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, Germany
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PTC Phage Technology Center GmbH, Siemensstraße 42, 59199 Bönen, Germany
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Robert Koch Institute, Burgstraße 37, 38855 Wernigerode, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Dann Turner
Viruses 2020, 12(12), 1470; https://doi.org/10.3390/v12121470
Received: 6 October 2020 / Revised: 11 December 2020 / Accepted: 16 December 2020 / Published: 19 December 2020
(This article belongs to the Section Bacterial Viruses)
Worldwide, poultry industry suffers from infections caused by avian pathogenic Escherichia coli. Therapeutic failure due to resistant bacteria is of increasing concern and poses a threat to human and animal health. This causes a high demand to find alternatives to fight bacterial infections in animal farming. Bacteriophages are being especially considered for the control of multi-drug resistant bacteria due to their high specificity and lack of serious side effects. Therefore, the study aimed on characterizing phages and composing a phage cocktail suitable for the prevention of infections with E. coli. Six phages were isolated or selected from our collections and characterized individually and in combination with regard to host range, stability, reproduction, and efficacy in vitro. The cocktail consisting of six phages was able to inhibit formation of biofilms by some E. coli strains but not by all. Phage-resistant variants arose when bacterial cells were challenged with a single phage but not when challenged by a combination of four or six phages. Resistant variants arising showed changes in carbon metabolism and/or motility. Genomic comparison of wild type and phage-resistant mutant E28.G28R3 revealed a deletion of several genes putatively involved in phage adsorption and infection. View Full-Text
Keywords: E. coli; bacteriophages; phage cocktail; colibacillosis; multidrug-resistant bacteria; biofilm; phage-resistant variants; APEC E. coli; bacteriophages; phage cocktail; colibacillosis; multidrug-resistant bacteria; biofilm; phage-resistant variants; APEC
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MDPI and ACS Style

Korf, I.H.E.; Kittler, S.; Bierbrodt, A.; Mengden, R.; Rohde, C.; Rohde, M.; Kroj, A.; Lehnherr, T.; Fruth, A.; Flieger, A.; Lehnherr, H.; Wittmann, J. In Vitro Evaluation of a Phage Cocktail Controlling Infections with Escherichia coli. Viruses 2020, 12, 1470. https://doi.org/10.3390/v12121470

AMA Style

Korf IHE, Kittler S, Bierbrodt A, Mengden R, Rohde C, Rohde M, Kroj A, Lehnherr T, Fruth A, Flieger A, Lehnherr H, Wittmann J. In Vitro Evaluation of a Phage Cocktail Controlling Infections with Escherichia coli. Viruses. 2020; 12(12):1470. https://doi.org/10.3390/v12121470

Chicago/Turabian Style

Korf, Imke H.E., Sophie Kittler, Anna Bierbrodt, Ruth Mengden, Christine Rohde, Manfred Rohde, Andrea Kroj, Tatiana Lehnherr, Angelika Fruth, Antje Flieger, Hansjörg Lehnherr, and Johannes Wittmann. 2020. "In Vitro Evaluation of a Phage Cocktail Controlling Infections with Escherichia coli" Viruses 12, no. 12: 1470. https://doi.org/10.3390/v12121470

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