Next Article in Journal
Emerging Role of PYHIN Proteins as Antiviral Restriction Factors
Next Article in Special Issue
Enhanced Cell-Based Detection of Parvovirus B19V Infectious Units According to Cell Cycle Status
Previous Article in Journal
Homo sapiens: The Superspreader of Plant Viral Diseases
Previous Article in Special Issue
Changes in Mass Treatment of the Canine Parvovirus ICU Population in Relation to Public Policy Changes during the COVID-19 Pandemic
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Viruses
Volume 12
Issue 12
10.3390/v12121463
Review Report
viruses-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

Printed Edition

A printed edition of this Special Issue is available at MDPI Books....
share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Review
Peer-Review Record

The VP1u of Human Parvovirus B19: A Multifunctional Capsid Protein with Biotechnological Applications

Viruses 2020, 12(12), 1463; https://doi.org/10.3390/v12121463
by Carlos Ros *, Jan Bieri and Remo Leisi
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Viruses 2020, 12(12), 1463; https://doi.org/10.3390/v12121463
Submission received: 2 December 2020 / Revised: 15 December 2020 / Accepted: 16 December 2020 / Published: 18 December 2020
(This article belongs to the Special Issue Advances in Parvovirus Research 2020)

Round 1

Reviewer 1 Report

The article is well written and the VP1u of parvovirus B 19 is described in great detail.

Here are several remarks:

Line 103: Four species of human bocaviruses are currently known and included in the Bocavirus genus. Please clarify it.

Figure 2. Could you add the proper bar scale?

Missing citing references for Figure 6.

Lines 130-132: Do the recombinant parvoviral particles remain VP1u?

Author Response

Reply to reviewer 1

Line 103: Four species of human bocaviruses are currently known and included in the Bocavirus genus. Please clarify it.

(HBoV) has been changed to (HBoV1-4).

Figure 2. Could you add the proper bar scale?

These are immunofluorescence images of methanol/acetone fixed cells. In our opinion, the size of the cells is irrelevant.

Missing citing references for Figure 6.

References have been included.

Lines 130-132: Do the recombinant parvoviral particles remain VP1u?

It is written "VP2 recombinant particles". Accordingly, these particles lack the entire VP1 (including VP1u).

Reviewer 2 Report

In this extensive review the authors give a general overview on Parvoviridae family, structure and replication, paying particular attention to human parvovirus B19 (B19V). Role of VP1u in restricted tropism of B19V as well in the subcellular trafficking of incoming virus are described in details, so giving explanations and background for the last section – biotechnological applications of the VP1u of B19V in diagnostic, drug delivery and chemotherapy of hematological diseases. The article is interesting and despite the fact that the material is relatively complex (especially for those who are not specialists in the field of parvoviruses) it is written in an understandable way and explained by data from previous publications.

In reality I have only one minor comment:

Line 248 – “Figure 4C, D and E” – there is no 4E in the Figure 4 (Lines 250-254).

Author Response

Reply to reviewer 2

Line 248 – “Figure 4C, D and E” – there is no 4E in the Figure 4 (Lines 250-254)

Error has been corrected

Back to TopTop