Next Article in Journal
Potentially Infectious Novel Hepatitis A Virus Strains Detected in Selected Treated Wastewater Discharge Sources, South Africa
Next Article in Special Issue
Completion of the AAV Structural Atlas: Serotype Capsid Structures Reveals Clade-Specific Features
Previous Article in Journal
An Amphipathic Alpha-Helix Domain from Poliovirus 2C Protein Tubulate Lipid Vesicles
Previous Article in Special Issue
The VP1u of Human Parvovirus B19: A Multifunctional Capsid Protein with Biotechnological Applications
 
 
Article

Enhanced Cell-Based Detection of Parvovirus B19V Infectious Units According to Cell Cycle Status

1
Laboratoire Français du Fractionnement et des Biotechnologies (LFB), 3 Avenue des Tropiques, BP 305, Courtabœuf CEDEX, 91958 Les Ulis, France
2
Division of Innovative Therapies, UMR-1184, IMVA-HB and IDMIT Center, CEA, INSERM and Paris-Saclay University, F-92265 Fontenay-aux-Roses, France
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Giorgio Gallinella
Viruses 2020, 12(12), 1467; https://doi.org/10.3390/v12121467
Received: 27 July 2020 / Revised: 21 September 2020 / Accepted: 29 September 2020 / Published: 18 December 2020
(This article belongs to the Special Issue Advances in Parvovirus Research 2020)
Human parvovirus B19 (B19V) causes various human diseases, ranging from childhood benign infection to arthropathies, severe anemia and fetal hydrops, depending on the health state and hematological status of the patient. To counteract B19V blood-borne contamination, evaluation of B19 DNA in plasma pools and viral inactivation/removal steps are performed, but nucleic acid testing does not correctly reflect B19V infectivity. There is currently no appropriate cellular model for detection of infectious units of B19V. We describe here an improved cell-based method for detecting B19V infectious units by evaluating its host transcription. We evaluated the ability of various cell lines to support B19V infection. Of all tested, UT7/Epo cell line, UT7/Epo-STI, showed the greatest sensitivity to B19 infection combined with ease of performance. We generated stable clones by limiting dilution on the UT7/Epo-STI cell line with graduated permissiveness for B19V and demonstrated a direct correlation between infectivity and S/G2/M cell cycle stage. Two of the clones tested, B12 and E2, reached sensitivity levels higher than those of UT7/Epo-S1 and CD36+ erythroid progenitor cells. These findings highlight the importance of cell cycle status for sensitivity to B19V, and we propose a promising new straightforward cell-based method for quantifying B19V infectious units. View Full-Text
Keywords: B19 parvovirus; detection; erythroid cells; cell cycle; permissivity B19 parvovirus; detection; erythroid cells; cell cycle; permissivity
Show Figures

Figure 1

MDPI and ACS Style

Ducloux, C.; You, B.; Langelé, A.; Goupille, O.; Payen, E.; Chrétien, S.; Kadri, Z. Enhanced Cell-Based Detection of Parvovirus B19V Infectious Units According to Cell Cycle Status. Viruses 2020, 12, 1467. https://doi.org/10.3390/v12121467

AMA Style

Ducloux C, You B, Langelé A, Goupille O, Payen E, Chrétien S, Kadri Z. Enhanced Cell-Based Detection of Parvovirus B19V Infectious Units According to Cell Cycle Status. Viruses. 2020; 12(12):1467. https://doi.org/10.3390/v12121467

Chicago/Turabian Style

Ducloux, Céline, Bruno You, Amandine Langelé, Olivier Goupille, Emmanuel Payen, Stany Chrétien, and Zahra Kadri. 2020. "Enhanced Cell-Based Detection of Parvovirus B19V Infectious Units According to Cell Cycle Status" Viruses 12, no. 12: 1467. https://doi.org/10.3390/v12121467

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop