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Review

Put Some Guts into It: Intestinal Organoid Models to Study Viral Infection

1
OrganoVIR Lab, Department of Medical Microbiology, Amsterdam UMC, Academic Medical Center, University of Amsterdam, 1100 AZ Amsterdam, The Netherlands
2
Department of Pediatrics Infectious Diseases, Emma Children’s Hospital, Amsterdam UMC, Academic Medical Center, University of Amsterdam, 1100 AZ Amsterdam, The Netherlands
*
Author to whom correspondence should be addressed.
Viruses 2020, 12(11), 1288; https://doi.org/10.3390/v12111288
Received: 28 September 2020 / Revised: 30 October 2020 / Accepted: 9 November 2020 / Published: 11 November 2020
(This article belongs to the Special Issue The Application of 3D Tissue Culture Systems in Virology)
The knowledge about enteric viral infection has vastly increased over the last eight years due to the development of intestinal organoids and enteroids that suppose a step forward from conventional studies using cell lines. Intestinal organoids and enteroids are three-dimensional (3D) models that closely mimic intestinal cellular heterogeneity and organization. The barrier function within these models has been adapted to facilitate viral studies. In this review, several adaptations (such as organoid-derived two-dimensional (2D) monolayers) and original intestinal 3D models are discussed. The specific advantages and applications, as well as improvements of each model are analyzed and an insight into the possible path for the field is given. View Full-Text
Keywords: intestinal organoid; enteroid; intestinal monolayer; Transwell®; Gut-on-a-Chip; Intestine-on-a-Chip; host-virus interactions; enteric virus intestinal organoid; enteroid; intestinal monolayer; Transwell®; Gut-on-a-Chip; Intestine-on-a-Chip; host-virus interactions; enteric virus
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MDPI and ACS Style

García-Rodríguez, I.; Sridhar, A.; Pajkrt, D.; Wolthers, K.C. Put Some Guts into It: Intestinal Organoid Models to Study Viral Infection. Viruses 2020, 12, 1288. https://doi.org/10.3390/v12111288

AMA Style

García-Rodríguez I, Sridhar A, Pajkrt D, Wolthers KC. Put Some Guts into It: Intestinal Organoid Models to Study Viral Infection. Viruses. 2020; 12(11):1288. https://doi.org/10.3390/v12111288

Chicago/Turabian Style

García-Rodríguez, Inés, Adithya Sridhar, Dasja Pajkrt, and Katja C. Wolthers. 2020. "Put Some Guts into It: Intestinal Organoid Models to Study Viral Infection" Viruses 12, no. 11: 1288. https://doi.org/10.3390/v12111288

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