Generation of a Reassortant Influenza A Subtype H3N2 Virus Expressing Gaussia Luciferase
AbstractReporter influenza A viruses (IAVs) carrying fluorescent or luminescent genes provide a powerful tool for both basic and translational research. Most reporter IAVs are based on the backbone of either subtype H1N1 viruses, A/Puerto Rico/8/1934 (PR8) or A/WSN/1933, but no reporter subtype H3N2 virus is currently available to our knowledge. Since the IAV subtype H3N2 co-circulates with H1N1 among humans causing annual epidemics, a reporter influenza A subtype H3N2 virus would be highly valuable. In this study, the segments of A/Wyoming/3/03 (NY, H3N2) virus encoding hemagglutinin and neuraminidase, respectively, were reassorted with the six internal genes of PR8 where the NS gene was fused with a Gaussia luciferase (Gluc) gene. Using reverse genetics, NY-r19-Gluc, a replication competent reassortant influenza A subtype H3N2 virus expressing reporter Gluc was successfully generated. This reporter virus is stable during replication in Madin-Darby canine kidney (MDCK) cells, and preliminary studies demonstrated it as a useful tool to evaluate antivirals. In addition, NY-r19-Gluc virus will be a powerful tool in other studies including the application of diagnostic and therapeutic antibodies as well as the evaluation of novel vaccines. View Full-Text
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Wang, L.; Cui, Q.; Zhao, X.; Li, P.; Wang, Y.; Rong, L.; Du, R. Generation of a Reassortant Influenza A Subtype H3N2 Virus Expressing Gaussia Luciferase. Viruses 2019, 11, 665.
Wang L, Cui Q, Zhao X, Li P, Wang Y, Rong L, Du R. Generation of a Reassortant Influenza A Subtype H3N2 Virus Expressing Gaussia Luciferase. Viruses. 2019; 11(7):665.Chicago/Turabian Style
Wang, Lin; Cui, Qinghua; Zhao, Xiujuan; Li, Ping; Wang, Yanyan; Rong, Lijun; Du, Ruikun. 2019. "Generation of a Reassortant Influenza A Subtype H3N2 Virus Expressing Gaussia Luciferase." Viruses 11, no. 7: 665.
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