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Open AccessArticle

Co-Expression of Chicken IL-2 and IL-7 Enhances the Immunogenicity and Protective Efficacy of a VP2-Expressing DNA Vaccine against IBDV in Chickens

Laboratory of Molecular Virology and Immunology, College of Animal Science and Technology/College of Veterinary Medicine, Hebei Agricultural University; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding 071000, Hebei, China
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Author to whom correspondence should be addressed.
These authors contributed equally to this paper.
Viruses 2019, 11(5), 476; https://doi.org/10.3390/v11050476
Received: 8 May 2019 / Revised: 22 May 2019 / Accepted: 23 May 2019 / Published: 24 May 2019
(This article belongs to the Section Antivirals & Vaccines)
Chicken infectious bursal disease (IBD) is still incompletely controlled worldwide. Although IBD virus (IBDV) VP2 DNA vaccine was considered a safe vaccine for IBD prevention, the immunogenicity by itself remains poor, resulting in the failure of effectively protecting chickens from infection. We and others demonstrated that chicken IL-2 (chIL-2) and chIL-7 have the capacity to enhance the immunogenicity of the VP2 DNA vaccine. However, whether chIL-2 and chIL-7 can mutually enhance the immunogenicity of VP2 DNA vaccine and thereby augment the latter’s protection efficacy remains unknown. By using chIL-2/chIL-7 bicistronic gene vector to co-immunize the chickens together with the VP2 DNA vaccine, we now show that chIL-2 and chIL-7 significantly increased IBDV VP2-specific antibody titers, T cell proliferation, and IFN-γ production, resulting in the ultimate enhancement of vaccine-induced protection efficacy relative to that of chIL-2 or chIL-7 gene vectors alone. These results suggest that chIL-2 and chIL-7 can mutually enhance VP2 DNA vaccine’s efficacy, thereby establishing a concrete foundation for future optimization of IBDV VP2 DNA vaccine to prevent/treat chicken IBD. View Full-Text
Keywords: chicken IL-2; chicken IL-7; mutual enhancement; immunogenicity; IBDV VP2 DNA vaccine chicken IL-2; chicken IL-7; mutual enhancement; immunogenicity; IBDV VP2 DNA vaccine
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Huo, S.; Zhang, J.; Fan, J.; Wang, X.; Wu, F.; Zuo, Y.; Zhong, F. Co-Expression of Chicken IL-2 and IL-7 Enhances the Immunogenicity and Protective Efficacy of a VP2-Expressing DNA Vaccine against IBDV in Chickens. Viruses 2019, 11, 476.

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