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New Bacteriophages against Emerging Lineages ST23 and ST258 of Klebsiella pneumoniae and Efficacy Assessment in Galleria mellonella Larvae

Bacteriology, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, ULiège, 4000 Liège, Belgium
Biodiversity and Epidemiology of Bacterial Pathogens, Institut Pasteur, 75015 Paris, France
Laboratory of Gene Technology, Department of Biosystems, KU Leuven, 3001 Heverlee, Belgium
Centre of Microbial and Plant Genetics, Department of Microbial and Molecular Systems, KU Leuven, 3001 Heverlee, Belgium
Department of Microbiology, Institut Pasteur, 75015 Paris, France
Laboratoire des Biomolécules, Hôpital Saint-Antoine, Sorbonne Université, 75012 Paris, France
Institute of Biology Leiden, Leiden University, 2311 Leiden, The Netherlands
Service de Réanimation Médico-Chirurgicale, Centre Hospitalier René Dubos, 95300 Pontoise, France
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Current Address: Department of Microbiology and Microbial Infection and Immunity, The Ohio State University, 43210 Columbus, OH, USA.
Viruses 2019, 11(5), 411;
Received: 14 February 2019 / Revised: 26 April 2019 / Accepted: 29 April 2019 / Published: 3 May 2019
(This article belongs to the Special Issue Hurdles for Phage Therapy (PT) to Become a Reality)
PDF [1451 KB, uploaded 3 May 2019]


Klebsiella pneumoniae is a bacterial pathogen of high public health importance. Its polysaccharide capsule is highly variable but only a few capsular types are associated with emerging pathogenic sublineages. The aim of this work is to isolate and characterize new lytic bacteriophages and assess their potential to control infections by the ST23 and ST258 K. pneumoniae sublineages using a Galleria mellonella larvae model. Three selected bacteriophages, targeting lineages ST258 (bacteriophages vB_KpnP_KL106-ULIP47 and vB_KpnP_KL106-ULIP54) and ST23 (bacteriophage vB_KpnP_K1-ULIP33), display specificity for capsular types KL106 and K1, respectively. These podoviruses belong to the Autographivirinae subfamily and their genomes are devoid of lysogeny or toxin-associated genes. In a G. mellonella larvae model, a mortality rate of 70% was observed upon infection by K. pneumoniae ST258 and ST23. This number was reduced to 20% upon treatment with bacteriophages at a multiplicity of infection of 10. This work increases the number of characterized bacteriophages infecting K. pneumoniae and provides information regarding genome sequence and efficacy during preclinical phage therapy against two prominent sublineages of this bacterial species. View Full-Text
Keywords: antimicrobial resistance; capsule; Galleria mellonella; Klebsiella pneumoniae; phage therapy antimicrobial resistance; capsule; Galleria mellonella; Klebsiella pneumoniae; phage therapy

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Thiry, D.; Passet, V.; Danis-Wlodarczyk, K.; Lood, C.; Wagemans, J.; De Sordi, L.; van Noort, V.; Dufour, N.; Debarbieux, L.; Mainil, J.G.; Brisse, S.; Lavigne, R. New Bacteriophages against Emerging Lineages ST23 and ST258 of Klebsiella pneumoniae and Efficacy Assessment in Galleria mellonella Larvae. Viruses 2019, 11, 411.

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