A Novel Hepacivirus in Wild Rodents from South America
Abstract
:1. Introduction
2. Materials and Methods
2.1. Rodent Samples and Ethical Statements
2.2. Viral RNA Extraction, Sequencing, and Assembly
2.3. Genome Characterization
2.4. Phylogenetic Analysis
2.5. p-Distance Analysis
2.6. Recombination Analysis
2.7. RT-PCR for Novel Hepacivirus
3. Results and Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Pool | Species | N1 | Collection Date | Number of Reads |
---|---|---|---|---|
1 | Akodon montensis | 55 | 2008 | 27,569,342 |
2 | Akodon montensis | 55 | 2008 | 23,439,326 |
3 | Akodon montensis | 41 | 2009 | 16,698,848 |
4 | Akodon montensis | 48 | 2012–2013 | 18,783,944 |
5 | Calomys tener | 38 | 2008 | 27,017,352 |
6 | Calomys tener | 37 | 2008 | 23,972,304 |
7 | Calomys tener | 34 | 2009, 2012–2013 | 15,679,756 |
8 | Necromys lasiurus | 59 | 2008 | 22,989,548 |
9 | Necromys lasiurus | 59 | 2008 | 9,252,300 |
10 | Necromys lasiurus | 58 | 2008 | 18,213,066 |
11 | Necromys lasiurus | 52 | 2009 | 22,210,888 |
12 | Necromys lasiurus | 24 | 2012–2013 | 25,122,228 |
13 | Oligoryzomys nigripes | 43 | 2008–2009 | 15,813,430 |
14 | Oligoryzomys nigripes | 18 | 2012–2013 | 24,796,054 |
15 | Mus musculus | 24 | 2008–2009 | 16,661,928 |
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de Souza, W.M.; Fumagalli, M.J.; Sabino-Santos, G., Jr.; Motta Maia, F.G.; Modha, S.; Teixeira Nunes, M.R.; Murcia, P.R.; Moraes Figueiredo, L.T. A Novel Hepacivirus in Wild Rodents from South America. Viruses 2019, 11, 297. https://doi.org/10.3390/v11030297
de Souza WM, Fumagalli MJ, Sabino-Santos G Jr., Motta Maia FG, Modha S, Teixeira Nunes MR, Murcia PR, Moraes Figueiredo LT. A Novel Hepacivirus in Wild Rodents from South America. Viruses. 2019; 11(3):297. https://doi.org/10.3390/v11030297
Chicago/Turabian Stylede Souza, William Marciel, Marcílio Jorge Fumagalli, Gilberto Sabino-Santos, Jr., Felipe Gonçalves Motta Maia, Sejal Modha, Márcio Roberto Teixeira Nunes, Pablo Ramiro Murcia, and Luiz Tadeu Moraes Figueiredo. 2019. "A Novel Hepacivirus in Wild Rodents from South America" Viruses 11, no. 3: 297. https://doi.org/10.3390/v11030297
APA Stylede Souza, W. M., Fumagalli, M. J., Sabino-Santos, G., Jr., Motta Maia, F. G., Modha, S., Teixeira Nunes, M. R., Murcia, P. R., & Moraes Figueiredo, L. T. (2019). A Novel Hepacivirus in Wild Rodents from South America. Viruses, 11(3), 297. https://doi.org/10.3390/v11030297