Next Article in Journal
A Needle in A Haystack: Tracing Bivalve-Associated Viruses in High-Throughput Transcriptomic Data
Next Article in Special Issue
Cell Cycle Arrest is a Conserved Function of Norovirus VPg Proteins
Previous Article in Journal
The Tug-of-War between Plants and Viruses: Great Progress and Many Remaining Questions
Previous Article in Special Issue
Antiviral Drug Discovery: Norovirus Proteases and Development of Inhibitors
Open AccessArticle

Genomics Analyses of GIV and GVI Noroviruses Reveal the Distinct Clustering of Human and Animal Viruses

Division of Viral Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA
Division of Microbiology, National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, USA
Department of Agriculture, School of Agriculture, Fisheries, and Human Sciences, University of Arkansas, Pine Bluff, AR 71601, USA
Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Centre, Giza 12622, Egypt
Author to whom correspondence should be addressed.
Viruses 2019, 11(3), 204;
Received: 31 January 2019 / Revised: 23 February 2019 / Accepted: 25 February 2019 / Published: 1 March 2019
(This article belongs to the Special Issue Noroviruses)
Noroviruses are highly diverse viruses that are the major viral cause of acute gastroenteritis in humans. Although these viruses can infect multiple mammalian species, their potential for zoonosis is not well understood, especially within Genogroup IV (GIV), which contains viruses that infect humans, canines, and felines. The study of GIV viruses has been, in part, hindered by the limited number of complete genomes. Here, we developed a full-genome amplicon-based platform that facilitated the sequencing of canine noroviruses circulating in the United States. Eight novel nearly full-length canine norovirus genomes and two nearly complete VP1 sequences, including four GIV.2, three GVI.1, and three GVI.2 viruses, were successfully obtained. Only animal strains exhibited GVI/GIV chimeric viruses, demonstrating restrictions in norovirus recombination. Using genomic, phylogenetic, and structural analyses, we show that differences within the major capsid protein and the non-structural proteins of GIV and GVI noroviruses could potentially limit cross-species transmission between humans, canines, and felines. View Full-Text
Keywords: norovirus; canine norovirus; inter-species transmission norovirus; canine norovirus; inter-species transmission
Show Figures

Figure 1

MDPI and ACS Style

Ford-Siltz, L.A.; Mullis, L.; Sanad, Y.M.; Tohma, K.; Lepore, C.J.; Azevedo, M.; Parra, G.I. Genomics Analyses of GIV and GVI Noroviruses Reveal the Distinct Clustering of Human and Animal Viruses. Viruses 2019, 11, 204.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop