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Viruses 2019, 11(2), 147; https://doi.org/10.3390/v11020147

Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in Aedes aegypti and Culex quinquefasciatus Mosquitoes from Brazil

1
Institute f Biological Sciences, Federal University of Pará, Av. Augusto Correa01, CEP 66075-000 Belém, Pará, Brazil
2
Laboratório de Vetores, Superintendência de Vigilância em Saúde do Amapá, Rua Tancredo Neves, 1.118, CEP 68905-230 Macapá, AP, Brazil
3
Lusíada University, Rua Oswaldo Cruz, 179, CEP 11045-101 Santos, SP, Brazil
4
Laboratório de Retrovirologia, Universidade Federal de São Paulo, Rua Pedro de Toledo, 781, CEP 04039-032 São Paulo, SP, Brazil
5
Faculty of Medicine of ABC, Santo André, SP 09060-870, Brazil
6
Department of Obstetrics and Gynecology, Weill Cornell Medicine, 407 E 61st St, New York, NY 10065, USA
7
Institute of Tropical Medicine, University of São Paulo, Avenida Dr. Enéas Carvalho de Aguiar, 470-CEP 05403-000 São Paulo, Brazil
8
Vitalant Research Institute, 270 Masonic Avenue, San Francisco, CA 94118-4417, USA
9
Department of Laboratory Medicine, University of California, Blood Systems Research Institute, 270 Masonic Ave, San Francisco, CA 94118, USA
10
Department of Infectious Diseases, Faculty of Medicine, University of São Paulo, Av. Dr. Arnaldo, 455-Cerqueira César CEP, 01246903 São Paulo, SP, Brazil
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 18 January 2019 / Revised: 5 February 2019 / Accepted: 8 February 2019 / Published: 10 February 2019
(This article belongs to the Special Issue Transmission Dynamics of Insect Viruses)
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Abstract

Advancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity of viruses (pathogens and non-pathogens) harbored by mosquitoes. Hubei reo-like virus 7 (HRLV 7) was recently detected by the virome analysis of fecal samples from migratory birds in Australia. We now report the detection of RNA-dependent RNA polymerase sequences of HRLV 7 in pools of Aedes aegypti and Culex quinquefasciatus mosquitoes species from the Brazilian Amazon forest. Phylogenetic inferences indicated that all HRLV 7 strains fall within the same independent clade. In addition, HRLV 7 shared a close ancestral lineage with the Dinovernavirus genus of the Reoviridae family. Our findings indicate that HRLV 7 is present in two species of mosquitoes. View Full-Text
Keywords: Hubei reo-like virus 7; reovirus; RNA-dependent RNA polymerase; metagenomic; Aedes aegypti; Culex quinquefasciatus; mosquitoes; insect-viruses; Amazon forest; arbovirus; birds; Brazil Hubei reo-like virus 7; reovirus; RNA-dependent RNA polymerase; metagenomic; Aedes aegypti; Culex quinquefasciatus; mosquitoes; insect-viruses; Amazon forest; arbovirus; birds; Brazil
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Ribeiro, G.O.; Monteiro, F.J.C.; Rego, M.O.S.; Ribeiro, E.S.D.; Castro, D.F.; Caseiro, M.M.; Souza Marinho, R.S.; Komninakis, S.V.; Witkin, S.S.; Deng, X.; Delwart, E.; Sabino, E.C.; da Costa, A.C.; Leal, É. Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in Aedes aegypti and Culex quinquefasciatus Mosquitoes from Brazil. Viruses 2019, 11, 147.

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