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Open AccessArticle

Use of Monocyte-Derived Macrophage Culture Increases Zika Virus Isolation Rate from Human Plasma

Laboratory of Emerging Pathogens (LEP), Division of Emerging Transfusion Transmitted diseases (DETTD), Office of Blood Research and Review (OBRR), Center for Biologics Evaluation and Research (CBER), Food and Drug Administration (FDA), Silver Spring, MD 20993, USA
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Viruses 2019, 11(11), 1058; https://doi.org/10.3390/v11111058
Received: 2 October 2019 / Revised: 11 November 2019 / Accepted: 13 November 2019 / Published: 14 November 2019
(This article belongs to the Special Issue Emerging Arboviruses)
Viral isolation is desirable for many reasons, including development of diagnostic assays and reference materials, and for virology basic research. Zika virus (ZIKV) isolation from clinical samples is challenging, but isolates are known to infect various cell lines. Here, we evaluated suitability of Vero, C6/36 and JEG-3 as host cells, for direct isolation of ZIKV from human plasma. We also assessed the use of primary monocyte-derived macrophages (MDMs) culture to enhance ZIKV isolation from human plasma samples followed by virus expansion in Vero, C6/36 and JEG-3 cultures. Direct inoculation of cell lines with 42 ZIKV-RNA positive samples resulted in isolation rates of 9.52% (4/42) in Vero and C6/36, and of 7.14% (3/42) in JEG-3 cells. Inoculation of plasma in MDMs followed by supernatant testing by TaqMan RT-PCR, resulted in 33/42 (78.57%) ZIKV-RNA-positive supernatants, which expansion in cell lines increased isolation rates to 24.24% (8/33) in Vero and to 27.27% (9/33) in C6/36 and JEG-3 regardless of the presence of ZIKV-antibody. Isolates generated in JEG-3 cells were also produced in Vero and C6/36 with similar viral titers. These results suggest that efficiency of ZIKV isolation from human plasma can be enhanced when MDM culture is used before viral expansion in cell lines. View Full-Text
Keywords: Zika virus; cell culture; primary isolation; in vitro model; infection Zika virus; cell culture; primary isolation; in vitro model; infection
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Sippert, E.; Rocha, B.C.; Assis, F.L.; Ok, S.; Rios, M. Use of Monocyte-Derived Macrophage Culture Increases Zika Virus Isolation Rate from Human Plasma. Viruses 2019, 11, 1058.

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