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Open AccessArticle

Genome Analysis of a Novel Clade II.b Alphabaculovirus Obtained from Artaxa digramma

State Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China
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Viruses 2019, 11(10), 925; https://doi.org/10.3390/v11100925
Received: 8 August 2019 / Revised: 30 September 2019 / Accepted: 7 October 2019 / Published: 9 October 2019
(This article belongs to the Special Issue Insect Viruses and Pest Management)
Artaxa digramma is a lepidopteran pest distributed throughout southern China, Myanmar, Indonesia, and India. Artaxa digramma nucleopolyhedrovirus (ArdiNPV) is a specific viral pathogen of A. digramma and deemed as a promising biocontrol agent against the pest. In this study, the complete genome sequence of ArdiNPV was determined by deep sequencing. The genome of ArdiNPV contains a double-stranded DNA (dsDNA) of 161,734 bp in length and 39.1% G+C content. Further, 149 hypothetical open reading frames (ORFs) were predicted to encode proteins >50 amino acids in length, covering 83% of the whole genome. Among these ORFs, 38 were baculovirus core genes, 22 were lepidopteran baculovirus conserved genes, and seven were unique to ArdiNPV, respectively. No typical baculoviral homologous regions (hrs) were identified in the genome. ArdiNPV had five multi-copy genes including baculovirus repeated ORFs (bros), calcium/sodium antiporter B (chaB), DNA binding protein (dbp), inhibitor of apoptosis protein (iap), and p26. Interestingly, phylogenetic analyses showed that ArdiNPV belonged to Clade II.b of Group II Alphabaculoviruses, which all contain a second copy of dbp. The genome of ArdiNPV was the closest to Euproctis pseudoconspersa nucleopolyhedrovirus, with 57.4% whole-genome similarity. Therefore, these results suggest that ArdiNPV is a novel baculovirus belonging to a newly identified cluster of Clade II.b Alphabaculoviruses. View Full-Text
Keywords: ArdiNPV; baculovirus; Clade II.b Alphabaculovirus ArdiNPV; baculovirus; Clade II.b Alphabaculovirus
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Li, J.; Duan, X.; Wang, Q.; Zhang, L.; Deng, F.; Wang, H.; Hu, Z.; Wang, M.; Wang, J. Genome Analysis of a Novel Clade II.b Alphabaculovirus Obtained from Artaxa digramma. Viruses 2019, 11, 925.

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