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Article

Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling Nanotubes

1
Université de Lyon, University Claude Bernard Lyon 1, INRA, EPHE, IVPC, UMR754, Viral Infections & Comparative Pathology, Cedex 07, 69366 Lyon, France
2
Institut de Biologie Structurale, CNRS UMR 5075, 38042 Grenoble, France
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Department of Medical Technology, School of Allied Health Sciences, University of Phayao, Phayao 56000, Thailand
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Center for Research & Innovation, Faculty of Medical Technology, Mahidol University, Nakhon Pathom 73170, Thailand
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EPHE, PSL Research University, INRA, Université de Lyon, University Claude Bernard Lyon 1, UMR754, IVPC, Cedex 07, 69366 Lyon, France
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UMR-1161 Virology, ANSES, INRA, Ecole Nationale Vétérinaire d’Alfort, ANSES Animal Health Laboratory, EURL on Equine Diseases, 94704 Maisons-Alfort, France
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Institut National de la Santé et de la Recherche Médicale, 101, rue de Tolbiac, Cedex 13, 75654 Paris, France
*
Author to whom correspondence should be addressed.
Viruses 2019, 11(10), 901; https://doi.org/10.3390/v11100901
Received: 1 August 2019 / Revised: 24 September 2019 / Accepted: 24 September 2019 / Published: 27 September 2019
(This article belongs to the Special Issue West Nile Virus 2019)
The cellular response to the recombinant NS1 protein of West Nile virus (NS1WNV) was studied using three different cell types: Vero E6 simian epithelial cells, SH-SY5Y human neuroblastoma cells, and U-87MG human astrocytoma cells. Cells were exposed to two different forms of NS1WNV: (i) the exogenous secreted form, sNS1WNV, added to the extracellular milieu; and (ii) the endogenous NS1WNV, the intracellular form expressed in plasmid-transfected cells. The cell attachment and uptake of sNS1WNV varied with the cell type and were only detectable in Vero E6 and SH-SY5Y cells. Addition of sNS1WNV to the cell culture medium resulted in significant remodeling of the actin filament network in Vero E6 cells. This effect was not observed in SH-SY5Y and U-87MG cells, implying that the cellular uptake of sNS1WNV and actin network remodeling were dependent on cell type. In the three cell types, NS1WNV-expressing cells formed filamentous projections reminiscent of tunneling nanotubes (TNTs). These TNT-like projections were found to contain actin and NS1WNV proteins. Interestingly, similar actin-rich, TNT-like filaments containing NS1WNV and the viral envelope glycoprotein EWNV were also observed in WNV-infected Vero E6 cells. View Full-Text
Keywords: flavivirus; West Nile virus; non-structural protein 1 (NS1); cytoskeleton; actin network; tunneling nanotubes flavivirus; West Nile virus; non-structural protein 1 (NS1); cytoskeleton; actin network; tunneling nanotubes
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MDPI and ACS Style

Furnon, W.; Fender, P.; Confort, M.-P.; Desloire, S.; Nangola, S.; Kitidee, K.; Leroux, C.; Ratinier, M.; Arnaud, F.; Lecollinet, S.; Boulanger, P.; Hong, S.-S. Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling Nanotubes. Viruses 2019, 11, 901. https://doi.org/10.3390/v11100901

AMA Style

Furnon W, Fender P, Confort M-P, Desloire S, Nangola S, Kitidee K, Leroux C, Ratinier M, Arnaud F, Lecollinet S, Boulanger P, Hong S-S. Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling Nanotubes. Viruses. 2019; 11(10):901. https://doi.org/10.3390/v11100901

Chicago/Turabian Style

Furnon, Wilhelm, Pascal Fender, Marie-Pierre Confort, Sophie Desloire, Sawitree Nangola, Kuntida Kitidee, Caroline Leroux, Maxime Ratinier, Frédérick Arnaud, Sylvie Lecollinet, Pierre Boulanger, and Saw-See Hong. 2019. "Remodeling of the Actin Network Associated with the Non-Structural Protein 1 (NS1) of West Nile Virus and Formation of NS1-Containing Tunneling Nanotubes" Viruses 11, no. 10: 901. https://doi.org/10.3390/v11100901

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