Infectious Bronchitis Virus Nonstructural Protein 4 Alone Induces Membrane Pairing
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells, Viruses, and Plasmids
2.2. SDS-PAGE and Western Blotting
2.3. Immunofluorescence Labelling
2.4. Transmission Electron Microscopy
2.5. Electron Tomography
3. Results
3.1. IBV Induces Typical Membrane Rearrangements in DF1 Cells
3.2. Non-Structural Proteins 3, 4, and 6 Relocalize upon Co-Expression
3.3. IBV nsp4 Alone Is Necessary and Sufficient to Induce Membrane Pairing
3.4. Nsp6 Induces the Formation of Piled, Disordered Membranes
3.5. Nsps 3, 4, and 6 Are Not Able to Recapitulate the IBV Replication Organelle
3.6. IBV nsp3 and 4 Do Not Induce DMVs
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Doyle, N.; Neuman, B.W.; Simpson, J.; Hawes, P.C.; Mantell, J.; Verkade, P.; Alrashedi, H.; Maier, H.J. Infectious Bronchitis Virus Nonstructural Protein 4 Alone Induces Membrane Pairing. Viruses 2018, 10, 477. https://doi.org/10.3390/v10090477
Doyle N, Neuman BW, Simpson J, Hawes PC, Mantell J, Verkade P, Alrashedi H, Maier HJ. Infectious Bronchitis Virus Nonstructural Protein 4 Alone Induces Membrane Pairing. Viruses. 2018; 10(9):477. https://doi.org/10.3390/v10090477
Chicago/Turabian StyleDoyle, Nicole, Benjamin W. Neuman, Jennifer Simpson, Philippa C. Hawes, Judith Mantell, Paul Verkade, Hasan Alrashedi, and Helena J. Maier. 2018. "Infectious Bronchitis Virus Nonstructural Protein 4 Alone Induces Membrane Pairing" Viruses 10, no. 9: 477. https://doi.org/10.3390/v10090477
APA StyleDoyle, N., Neuman, B. W., Simpson, J., Hawes, P. C., Mantell, J., Verkade, P., Alrashedi, H., & Maier, H. J. (2018). Infectious Bronchitis Virus Nonstructural Protein 4 Alone Induces Membrane Pairing. Viruses, 10(9), 477. https://doi.org/10.3390/v10090477