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Viruses 2018, 10(9), 450; https://doi.org/10.3390/v10090450

Sequence Analysis of Plum pox virus Strain C Isolates from Russia Revealed Prevalence of the D96E Mutation in the Universal Epitope and Interstrain Recombination Events

1
Department of Virology, Faculty of Biology, Lomonosov Moscow State University, Moscow 119234, Russia
2
Tatar Research Institute of Agriculture, Kazan 420059, Russia
*
Author to whom correspondence should be addressed.
Received: 10 July 2018 / Revised: 13 August 2018 / Accepted: 22 August 2018 / Published: 23 August 2018
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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Abstract

The understanding of genetic diversity, geographic distribution, and antigenic properties of Plum pox virus (PPV) is a prerequisite to improve control of sharka, the most detrimental viral disease of stone fruit crops worldwide. Forty new PPV strain C isolates were detected in sour cherry (Prunus cerasus) from three geographically distant (700–1100 km) regions of European Russia. Analysis of their 3’-terminal genomic sequences showed that nineteen isolates (47.5%) bear the D96E mutation in the universal epitope of the coat protein. Almost all of them cannot be detected by the monoclonal antibody 5B in triple antibody sandwich enzyme-linked immunosorbent assay and Western blot analysis that may potentially compromise serological PPV detection in cherries. Full-length genomes of seven PPV-C isolates were determined employing next-generation sequencing. Using the Recombination Detection Program (RDP4), the recombination event covering the region from (Cter)P1 to the middle of the HcPro gene was predicted in all the available PPV-C complete genomes. The isolates Tat-4, belonging to the strain CV, and RU-17sc (PPV-CR) were inferred as major and minor parents, respectively, suggesting possible pathways of evolution of the cherry-adapted strains. Downy cherry (P. tomentosa) was identified as the natural PPV-C host for the first time. View Full-Text
Keywords: Plum pox virus; sour cherry; strain C; next-generation sequencing; recombination; seed transmission Plum pox virus; sour cherry; strain C; next-generation sequencing; recombination; seed transmission
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Sheveleva, A.; Ivanov, P.; Gasanova, T.; Osipov, G.; Chirkov, S. Sequence Analysis of Plum pox virus Strain C Isolates from Russia Revealed Prevalence of the D96E Mutation in the Universal Epitope and Interstrain Recombination Events. Viruses 2018, 10, 450.

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