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Viruses 2018, 10(6), 279; https://doi.org/10.3390/v10060279

Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function

1
The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, Pirbright, Ash Road, Guildford, Surrey GU24 0NF, UK
2
Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases, Binzhou 256600, Shandong, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 27 April 2018 / Revised: 17 May 2018 / Accepted: 22 May 2018 / Published: 24 May 2018
(This article belongs to the Section Animal Viruses)
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Abstract

Marek’s disease virus (MDV) is a member of alphaherpesviruses associated with Marek’s disease, a highly contagious neoplastic disease in chickens. Complete sequencing of the viral genome and recombineering techniques using infectious bacterial artificial chromosome (BAC) clones of Marek’s disease virus genome have identified major genes that are associated with pathogenicity. Recent advances in CRISPR/Cas9-based gene editing have given opportunities for precise editing of the viral genome for identifying pathogenic determinants. Here we describe the application of CRISPR/Cas9 gene editing approaches to delete the Meq and pp38 genes from the CVI988 vaccine strain of MDV. This powerful technology will speed up the MDV gene function studies significantly, leading to a better understanding of the molecular mechanisms of MDV pathogenesis. View Full-Text
Keywords: CRISPR/Cas9; CVI988; Meq; pp38; deletion CRISPR/Cas9; CVI988; Meq; pp38; deletion
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Zhang, Y.; Tang, N.; Sadigh, Y.; Baigent, S.; Shen, Z.; Nair, V.; Yao, Y. Application of CRISPR/Cas9 Gene Editing System on MDV-1 Genome for the Study of Gene Function. Viruses 2018, 10, 279.

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