Next Article in Journal
All-Round Manipulation of the Actin Cytoskeleton by HIV
Previous Article in Journal
Virus–Bacteria Interactions: Implications and Potential for the Applied and Agricultural Sciences
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessArticle
Viruses 2018, 10(2), 62; https://doi.org/10.3390/v10020062

A Simple Mechanism Based on Amino Acid Substitutions is not a Critical Determinant of High Mortality of Japanese Encephalitis Virus Infection in Mice

1
Department of Virology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
2
Leading Graduate School Program, Nagasaki University, Nagasaki 852-8523, Japan
3
Center for Control and Prevention of Infectious Diseases, Nagasaki University, Nagasaki 852-8523, Japan
Current Affiliation: Institute of Virology, Philipps University Marburg, 35037 Marburg, Germany.
Current Affiliation: Laboratory of Zoonotic Diseases, Division of Health and Environmental Science, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu 069-8501, Japan.
*
Author to whom correspondence should be addressed.
Received: 22 December 2017 / Revised: 24 January 2018 / Accepted: 1 February 2018 / Published: 3 February 2018
(This article belongs to the Section Animal Viruses)
Full-Text   |   PDF [1507 KB, uploaded 6 February 2018]   |  

Abstract

For the development of effective treatment strategies for Japanese encephalitis (JE), it is important to identify the viral factors causing severe disease during JE virus (JEV) infection. In this study, we assessed whether amino acid substitutions are critical factors for higher mortality of JaTH160 compared with JaOArS982 in mice using the technique of infectious cDNA clones. We raised the possibility that two amino acids of C124 and NS3482 of JaTH160 may contribute to increased mortality in mice. However, simultaneous substitutions of these amino acids did not significantly increase the virulence of JaOArS982, suggesting that high mortality due to JaTH160 viral infection cannot be simply attributed to the specific amino acids. Multiple and complex, but not simple, mechanisms may induce the high mortality of JaTH160 infection in mice. View Full-Text
Keywords: Japanese encephalitis virus; pathogenicity; mouse; recombinant; amino acid substitution Japanese encephalitis virus; pathogenicity; mouse; recombinant; amino acid substitution
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Takamatsu, Y.; Uchida, L.; Raekiansyah, M.; Luz, M.A.; Morita, K.; Hayasaka, D. A Simple Mechanism Based on Amino Acid Substitutions is not a Critical Determinant of High Mortality of Japanese Encephalitis Virus Infection in Mice. Viruses 2018, 10, 62.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top