Next Article in Journal
Virus Infection Triggers MAVS Polymers of Distinct Molecular Weight
Next Article in Special Issue
Rapid CRISPR/Cas9-Mediated Cloning of Full-Length Epstein-Barr Virus Genomes from Latently Infected Cells
Previous Article in Journal
Optimizing the Targeting of Mouse Parvovirus 1 to Murine Melanoma Selects for Recombinant Genomes and Novel Mutations in the Viral Capsid Gene
Previous Article in Special Issue
CRISPR/Cas9—Advancing Orthopoxvirus Genome Editing for Vaccine and Vector Development
Article Menu
Issue 2 (February) cover image

Export Article

Open AccessReview
Viruses 2018, 10(2), 55;

CRISPR–Cas9 Genetic Analysis of Virus–Host Interactions

Harvard Program in Virology, Harvard Medical School, Boston, MA 02115, USA
Division of Infectious Disease, Department of Medicine, Brigham & Women’s Hospital, Boston, MA 02115, USA
Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 02115, USA
Broad Institute of Harvard and Massachusetts Institute of Technology, Cambridge, MA 02142, USA
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 13 January 2018 / Revised: 28 January 2018 / Accepted: 29 January 2018 / Published: 30 January 2018
(This article belongs to the Special Issue Applications of CRISPR Technology in Virology 2018)
Full-Text   |   PDF [1845 KB, uploaded 30 January 2018]   |  


Clustered regularly interspaced short palindromic repeats (CRISPR) has greatly expanded the ability to genetically probe virus–host interactions. CRISPR systems enable focused or systematic, genomewide studies of nearly all aspects of a virus lifecycle. Combined with its relative ease of use and high reproducibility, CRISPR is becoming an essential tool in studies of the host factors important for viral pathogenesis. Here, we review the use of CRISPR–Cas9 for the loss-of-function analysis of host dependency factors. We focus on the use of CRISPR-pooled screens for the systematic identification of host dependency factors, particularly in Epstein–Barr virus-transformed B cells. We also discuss the use of CRISPR interference (CRISPRi) and gain-of-function CRISPR activation (CRISPRa) approaches to probe virus–host interactions. Finally, we comment on the future directions enabled by combinatorial CRISPR screens. View Full-Text
Keywords: genome engineering; CRISPR screen; Cas9; Epstein–Barr virus; host dependency factor; CRISPR interference; CRISPR activation; combinatorial CRISPR genome engineering; CRISPR screen; Cas9; Epstein–Barr virus; host dependency factor; CRISPR interference; CRISPR activation; combinatorial CRISPR

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Gebre, M.; Nomburg, J.L.; Gewurz, B.E. CRISPR–Cas9 Genetic Analysis of Virus–Host Interactions. Viruses 2018, 10, 55.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Viruses EISSN 1999-4915 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top