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Open AccessArticle

Genomic Analysis of the Recent Viral Isolate vB_BthP-Goe4 Reveals Increased Diversity of φ29-Like Phages

1
Department of Genomic and Applied Microbiology & Göttingen Genomics Laboratory, Institute of Microbiology and Genetics, Georg-August-University Göttingen, 37077 Göttingen, Germany
2
Department of General Microbiology, Institute of Microbiology and Genetics, Georg-August-University Göttingen, 37077 Göttingen, Germany
*
Author to whom correspondence should be addressed.
Viruses 2018, 10(11), 624; https://doi.org/10.3390/v10110624
Received: 19 October 2018 / Revised: 6 November 2018 / Accepted: 8 November 2018 / Published: 13 November 2018
(This article belongs to the Special Issue Viruses of Microbes V: Biodiversity and Future Applications)
We present the recently isolated virus vB_BthP-Goe4 infecting Bacillus thuringiensis HD1. Morphological investigation via transmission electron microscopy revealed key characteristics of the genus Phi29virus, but with an elongated head resulting in larger virion particles of approximately 50 nm width and 120 nm height. Genome sequencing and analysis resulted in a linear phage chromosome of approximately 26 kb, harbouring 40 protein-encoding genes and a packaging RNA. Sequence comparison confirmed the relation to the Phi29virus genus and genomes of other related strains. A global average nucleotide identity analysis of all identified φ29-like viruses revealed the formation of several new groups previously not observed. The largest group includes Goe4 and may significantly expand the genus Phi29virus (Salasvirus) or the Picovirinae subfamily. View Full-Text
Keywords: Bacillus; thuringiensis; vB_BthP-Goe4; Goe4; Picovirinae; Phi29virus; Salasvirus; Luci; bacteriophage; phage; pRNA Bacillus; thuringiensis; vB_BthP-Goe4; Goe4; Picovirinae; Phi29virus; Salasvirus; Luci; bacteriophage; phage; pRNA
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Schilling, T.; Hoppert, M.; Hertel, R. Genomic Analysis of the Recent Viral Isolate vB_BthP-Goe4 Reveals Increased Diversity of φ29-Like Phages. Viruses 2018, 10, 624.

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