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Open AccessArticle

Impact of Two Reoviruses and Their Coinfection on the Rice RNAi System and vsiRNA Production

by Zhanbiao Li 1,2, Tong Zhang 1,2, Xiuqin Huang 1,2 and Guohui Zhou 1,2,*
1
Guangdong Province Key Laboratory of Microbial Signals and Disease Control, College of Agriculture, South China Agricultural University, Guangzhou 510642, China
2
Integrative Microbiology Research Center, South China Agricultural University, Guangzhou 510642, China
*
Author to whom correspondence should be addressed.
Viruses 2018, 10(11), 594; https://doi.org/10.3390/v10110594
Received: 7 September 2018 / Revised: 20 October 2018 / Accepted: 27 October 2018 / Published: 30 October 2018
(This article belongs to the Special Issue Plant-Virus Interactions)
Both Southern rice black-streaked dwarf virus (SRBSDV) and Rice ragged stunt virus (RRSV) belong to the family Reoviridae, and synergistic infection of these two viruses commonly occurs in the field. This study revealed that both SRBSDV and RRSV affect the RNA interference (RNAi) pathway and form different virus-derived interfering RNA (vsiRNA) profiles in rice. Co-infection of rice by SRBSDV and RRSV up-regulated the expression of rice DICER-like (DCL) proteins but down-regulated the expression of rice RNA-dependent RNA polymerases (RDRs), and the accumulation of vsiRNAs of either RBSDV or RRSV was decreased compared with that in singly infected plants. The majority of SRBSDV vsiRNAs were 21 nt or 22 nt in length, whether plants were singly infected with SRBSDV or co-infected with RRSV. On the other hand, the majority of RRSV vsiRNAs were 20 nt, 21 nt, or 22 nt in length, among which those 20 nt in length accounted for the largest proportion; co-infection with SRBSDV further increased the proportion of 20 nt vsiRNAs and decreased the proportion of 21 nt vsiRNAs. Co-infection had no effects on the strand favoritism and hot spots of the vsiRNAs, but changed the bias of the 5′ terminal nucleotide significantly. This study provides a reference for further study on the pathogenesis and synergistic mechanism of SRBSDV and RRSV. View Full-Text
Keywords: southern rice black-streaked dwarf virus (SRBSDV); rice ragged stunt virus (RRSV); synergism; RNA interference (RNAi); virus-derived interfering RNAs (vsiRNAs) southern rice black-streaked dwarf virus (SRBSDV); rice ragged stunt virus (RRSV); synergism; RNA interference (RNAi); virus-derived interfering RNAs (vsiRNAs)
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MDPI and ACS Style

Li, Z.; Zhang, T.; Huang, X.; Zhou, G. Impact of Two Reoviruses and Their Coinfection on the Rice RNAi System and vsiRNA Production. Viruses 2018, 10, 594.

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