Breed Differences in PCV2 Uptake and Disintegration in Porcine Monocytes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Peripheral Blood Monocytes
2.2. Virus Preparation and Particle Quantification
2.3. The Fate of PCV2 Capsids in Blood Monocytes
2.4. Effect of Different Entry Inhibitors on PCV2 Uptake
2.5. PCV2 Trafficking in Blood Monocytes
2.6. The Fate of PCV2 Viral Genomes in Blood Monocytes
2.7. In Vitro Test of the Ability of Lysosomal Deoxyribonuclease II (DNase II) to Degrade PCV2 Genome
2.8. Kinetics of PCV2 Uptake and Disintegration in Blood Monocytes from Four Pig Breeds
2.9. Statistical Analysis
3. Results
3.1. The Uptake and Disintegration of PCV2 Capsids in Blood Monocytes
3.2. PCV2 Is Taken up by Blood Monocytes via Clathrin-Mediated Endocytosis
3.3. The Uptake of PCV2 by Blood Monocytes Requires Actin and Dynamin
3.4. PCV2 Traffics Along the Endosome-Lysosome Pathway
3.5. PCV2 Genome Persists in Blood Monocytes in Its Complete Form
3.6. Deoxyribonuclease II (DNase II) from Porcine Lysosome can Degrade PCV2 Genome
3.7. Blood Monocytes Differ in the Ability to Take up and Disintegrate PCV2
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Primer Set | Forward Primer (5′ to 3′) | Reverse Primer (5′ to 3′) | Product |
wgPCV2-fw/rev | ggaagcttcagtaatttatttcatatggaa | ggaagcttttttatcacttcgtaatggtt | 1767 bp (whole PCV2 genome) |
ORF2-fw/rev | gcgcacttcttttcgttttcag | gaatgcggccgcttatcacttcgtaatggtttttattattca | 756 bp (DNase II cleavage site included) |
pgPCV2-fw/rev | ggctgtggcctttgktac | tgtrgaccacgtaggcctcg | 1598 bp (DNase II cleavage site not included) |
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Wei, R.; Trus, I.; Yang, B.; Huang, L.; Nauwynck, H.J. Breed Differences in PCV2 Uptake and Disintegration in Porcine Monocytes. Viruses 2018, 10, 562. https://doi.org/10.3390/v10100562
Wei R, Trus I, Yang B, Huang L, Nauwynck HJ. Breed Differences in PCV2 Uptake and Disintegration in Porcine Monocytes. Viruses. 2018; 10(10):562. https://doi.org/10.3390/v10100562
Chicago/Turabian StyleWei, Ruifang, Ivan Trus, Bo Yang, Liping Huang, and Hans J. Nauwynck. 2018. "Breed Differences in PCV2 Uptake and Disintegration in Porcine Monocytes" Viruses 10, no. 10: 562. https://doi.org/10.3390/v10100562