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Article

Identification and Functional Evaluation of Three Polyubiquitin Promoters from Hevea brasiliensis

1
Key Laboratory of Biology and Genetic Resources of Rubber Tree, Ministry of Agriculture and Rural Affairs, State Key Laboratory Incubation Base for Cultivation & Physiology of Tropical Crops, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
2
Haikou Key Laboratory of Innovation of Seedlings of Tropical Plants, Haikou 571101, China
*
Author to whom correspondence should be addressed.
Academic Editors: Giovanni Emiliani and Alessio Giovannelli
Forests 2022, 13(6), 952; https://doi.org/10.3390/f13060952
Received: 11 April 2022 / Revised: 24 May 2022 / Accepted: 15 June 2022 / Published: 17 June 2022
(This article belongs to the Special Issue Tree Genetics: Molecular and Functional Characterization of Genes)
Hevea brasiliensis is an economically important tree species that provides the only commercial source of natural rubber. The replacement of the CaMV35S promoter by endogenous polyubiquitin promoters may be a viable way to improve the genetic transformation of this species. However, no endogenous polyubiquitin promoters in Hevea have been reported yet. Here, we identified three Hevea polyubiquitin genes HbUBI10.1, HbUBI10.2 and HbUBI10.3, which encode ubiquitin monomers having nearly identical amino acid sequences to that of AtUBQ10. The genomic fragments upstream of these HbUBI genes, including the signature leading introns, were amplified as putative HbUBI promoters. In silico analysis showed that a number of cis-acting elements which are conserved within strong constitutive polyubiquitin promoters were presented in these HbUBI promoters. Transcriptomic data revealed that HbUBI10.1 and HbUBI10.2 had a constitutive expression in Hevea plants. Semi-quantitative RT-PCR showed that these three HbUBI genes were expressed higher than the GUS gene driven by CaMV35S in transgenic Hevea leaves. All three HbUBI promoters exhibited the capability to direct GFP expression in both transient and stable transformation assays, although they produced lower protoplast transformation efficiencies than the CaMV35S promoter. These HbUBI promoters will expand the availability of promoters for driving the transgene expression in Hevea genetic engineering. View Full-Text
Keywords: rubber tree; constitutive promoter; cis-acting elements; Agrobacterium-mediated transformation; protoplast transformation; transgene expression rubber tree; constitutive promoter; cis-acting elements; Agrobacterium-mediated transformation; protoplast transformation; transgene expression
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MDPI and ACS Style

Xin, S.; Udayabhanu, J.; Dai, X.; Hua, Y.; Fan, Y.; Huang, H.; Huang, T. Identification and Functional Evaluation of Three Polyubiquitin Promoters from Hevea brasiliensis. Forests 2022, 13, 952. https://doi.org/10.3390/f13060952

AMA Style

Xin S, Udayabhanu J, Dai X, Hua Y, Fan Y, Huang H, Huang T. Identification and Functional Evaluation of Three Polyubiquitin Promoters from Hevea brasiliensis. Forests. 2022; 13(6):952. https://doi.org/10.3390/f13060952

Chicago/Turabian Style

Xin, Shichao, Jinu Udayabhanu, Xuemei Dai, Yuwei Hua, Yueting Fan, Huasun Huang, and Tiandai Huang. 2022. "Identification and Functional Evaluation of Three Polyubiquitin Promoters from Hevea brasiliensis" Forests 13, no. 6: 952. https://doi.org/10.3390/f13060952

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