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Article

Micropropagation, Characterization, and Conservation of Phytophthora cinnamomi-Tolerant Holm Oak Mature Trees

1
Instituto de Investigaciones Agrobiológicas de Galicia (IIAG), Avda. Vigo s/n, 15705 Santiago de Compostela, Spain
2
Departamento Biología Vegetal, Instituto Biotec/Med, Facultad de Farmacia, Universitat de València, Avda, Vicent Andrés Estellés s/n, 46100 Burjassot, Spain
3
Departamento Ciencias Agrarias y del Medio Natural, Instituto Universitario de Ciencias Ambientales (IUCA), Universidad de Zaragoza, Escuela Politécnica Superior, Ctra. Cuarte s/n, 22071 Huesca, Spain
*
Author to whom correspondence should be addressed.
Academic Editors: Jorge Canhoto, Paloma Moncaleán and Sandra Correia
Forests 2021, 12(12), 1634; https://doi.org/10.3390/f12121634 (registering DOI)
Received: 11 October 2021 / Revised: 9 November 2021 / Accepted: 19 November 2021 / Published: 25 November 2021
(This article belongs to the Special Issue Application of Biotechnology Techniques on Tree Species)
Holm oak populations have deteriorated drastically due to oak decline syndrome. The first objective of the present study was to investigate the use of axillary budding and somatic embryogenesis (SE) to propagate asymptomatic holm oak genotypes identified in disease hotspots in Spain. Axillary budding was achieved in two out of six tolerant genotypes from the south-western region and in two out of four genotypes from the Mediterranean region. Rooting of shoots cultured on medium supplemented with 3 mg L−1 of indole-3-acetic acid plus 0.1 mg L−1 α-naphthalene acetic acid was achieved, with rates ranging from 8 to 36%. Shoot cultures remained viable after cold storage for 9–12 months; this procedure is therefore suitable for medium-term conservation of holm oak germplasm. SE was induced in two out of the three genotypes tested, by using nodes and shoot tips cultured in medium without plant growth regulators. In vitro cloned progenies of the tolerant genotypes PL-T2 and VA5 inhibited growth of Phytophthora cinnamomi mycelia when exposed to the oomycete in vitro. Significant differences in total phenol contents and in the expression profiles of genes regulating phenylpropanoid biosynthesis were observed between in vitro cultured shoots derived from tolerant trees and cultures established from control genotypes. View Full-Text
Keywords: axillary budding; disease-tolerant trees; dual cultures; gene expression; in vitro conservation; oak decline; Quercus ilex; phenols; Phytophthora cinnamomi; somatic embryogenesis axillary budding; disease-tolerant trees; dual cultures; gene expression; in vitro conservation; oak decline; Quercus ilex; phenols; Phytophthora cinnamomi; somatic embryogenesis
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MDPI and ACS Style

Martínez, M.T.; Arrillaga, I.; Sales, E.; Pérez-Oliver, M.A.; González-Mas, M.d.C.; Corredoira, E. Micropropagation, Characterization, and Conservation of Phytophthora cinnamomi-Tolerant Holm Oak Mature Trees. Forests 2021, 12, 1634. https://doi.org/10.3390/f12121634

AMA Style

Martínez MT, Arrillaga I, Sales E, Pérez-Oliver MA, González-Mas MdC, Corredoira E. Micropropagation, Characterization, and Conservation of Phytophthora cinnamomi-Tolerant Holm Oak Mature Trees. Forests. 2021; 12(12):1634. https://doi.org/10.3390/f12121634

Chicago/Turabian Style

Martínez, Mª T., Isabel Arrillaga, Ester Sales, María A. Pérez-Oliver, Mª d.C. González-Mas, and Elena Corredoira. 2021. "Micropropagation, Characterization, and Conservation of Phytophthora cinnamomi-Tolerant Holm Oak Mature Trees" Forests 12, no. 12: 1634. https://doi.org/10.3390/f12121634

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