PmMYB4, a Transcriptional Activator from Pinus massoniana, Regulates Secondary Cell Wall Formation and Lignin Biosynthesis
Abstract
:1. Introduction
2. Results
2.1. Isolation and Characterization of PmMYB4
2.2. PmMYB4 Is Primarily Expressed in Bark
2.3. Transcriptional Activation of PmMYB4 and the Binding Ability of PmMYB4 with AC Elements
2.4. Growth and Morphological Characteristics of PmMYB4 Transgenic Tobacco
2.5. Overexpression of PmMYB4 Positively Regulates SCW in Transgenic Tobacco
2.6. Overexpression of PmMYB4 Affects the Expression of SCW Biosynthesis Genes in Transgenic Tobacco
2.7. The PmPAL and PmCCoAOMT Promoters Are Activated by PmMYB4
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Stress Treatments
4.2. Cloning of the Full-Length PmMYB4 Coding Sequence (CDS) and Promoter
4.3. Sequence and Phylogenetic Analysis
4.4. Transcriptional Activation Analysis of PmMYB4
4.5. Semiquantitative RT-PCR and Quantitative Real-Time PCR
4.6. Agrobacterium-Mediated Transformation of Tobacco
4.7. Plant Height and Biomass Measurements
4.8. Microscopy and Histochemistry
4.9. Measurement of Lignin, Cellulose, and Hemicellulose Components in Transgenic Tobacco
4.10. Transient Expression and LUC/GUS Activity Assay
4.11. Statistical Analysis
4.12. GenBank Accession Numbers of Genes Used in This Study
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Samples | Lignin (%) | Cellulose (%) | Hemicellulose (%) |
---|---|---|---|
WT | 21.56 ± 0.16 | 42.17 ± 0.92 | 24.56 ± 1.0 |
L1 | 22.80 ± 0.11 * | 42.38 ± 0.97 | 25.41 ± 1.7 |
L5 | 25.09 ± 0.32 * | 44.56 ± 0.36 * | 24.16 ± 2.8 |
L6 | 24.80 ± 0.11 * | 44.34 ± 0.97 * | 24.45 ± 1.3 |
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Yao, S.; Chen, P.; Yu, Y.; Zhang, M.; Wang, D.; Liu, J.; Hao, Q.; Ji, K. PmMYB4, a Transcriptional Activator from Pinus massoniana, Regulates Secondary Cell Wall Formation and Lignin Biosynthesis. Forests 2021, 12, 1618. https://doi.org/10.3390/f12121618
Yao S, Chen P, Yu Y, Zhang M, Wang D, Liu J, Hao Q, Ji K. PmMYB4, a Transcriptional Activator from Pinus massoniana, Regulates Secondary Cell Wall Formation and Lignin Biosynthesis. Forests. 2021; 12(12):1618. https://doi.org/10.3390/f12121618
Chicago/Turabian StyleYao, Sheng, Peizhen Chen, Ye Yu, Mengyang Zhang, Dengbao Wang, Jiahe Liu, Qingqing Hao, and Kongshu Ji. 2021. "PmMYB4, a Transcriptional Activator from Pinus massoniana, Regulates Secondary Cell Wall Formation and Lignin Biosynthesis" Forests 12, no. 12: 1618. https://doi.org/10.3390/f12121618