Next Article in Journal
Nitrogen Deposition and Responses of Forest Structure to Nitrogen Deposition in a Cool-Temperate Deciduous Forest
Previous Article in Journal
Assessing Forest Structure and Composition along the Altitudinal Gradient in the State of Sikkim, Eastern Himalayas, India
Open AccessArticle

Hypocrealean Fungi Associated with Hylobius abietis in Slovakia, Their Virulence against Weevil Adults and Effect on Feeding Damage in Laboratory

Institute of Forest Ecology, Slovak Academy of Sciences, Akademická 2, 949 01 Nitra, Slovak Republic
National Forest Centre, Forest Research Institute Zvolen, T.G. Masaryka 22, 960 01 Zvolen, Slovak Republic
Department of Forest Protection and Entomology, Faculty of Forestry and Wood Sciences, Czech University of Life Sciences Prague, Kamýcká 1176, 165 00 Prague 6, Czech Republic
Research Station of State Forests of TANAP, 059 60 Tatranská Lomnica, Slovak Republic
Author to whom correspondence should be addressed.
Forests 2019, 10(8), 634;
Received: 4 July 2019 / Revised: 23 July 2019 / Accepted: 25 July 2019 / Published: 27 July 2019
(This article belongs to the Section Forest Ecology and Management)
In temperate regions of Europe, the large pine weevil, Hylobius abietis, is a major pest of coniferous forests mostly at sites where clear-felling is followed by planting of saplings. Control measures against this pest are based on silvicultural techniques, an application of physical barriers on stems of saplings and insecticide treatments. To avoid the use of insecticides, alternative measures such as biological control have been investigated. The goal of the present study was to obtain local strains of entomopathogenic fungi (Ascomycota, Hypocreales) from natural populations of H. abietis, and to investigate their efficacy against the weevil. A survey on entomopathogenic fungi was undertaken at clear-felled areas of spruce forests in northern Slovakia. Two Beauveria species, B. bassiana and B. pseudobassiana, were identified, and 22 in vitro strains were obtained. Mean prevalence of infected adults was low (2.10% ± 0.67%) and the mycosis was mostly recorded during May and June. Virulence of Beauveria strains against the weevil was tested in laboratory. B. bassiana strain AMEP20 was significantly most virulent (LC50 of 0.65 ± 0.10 × 108 conidia mL−1). Treatment with conidia of AMEP20 strain affected feeding damage by the weevil on bark of Scots pine twigs. Daily bark consumption by B. bassiana-treated weevils was lower than by untreated individuals and decreased with increasing conidia concentration used for the treatment. In the outdoor experiment, AMEP20 strain killed weevils that fed on spruce saplings treated with conidia suspensions. Mortality due to mycosis on weevils exposed to the conidia-treated saplings reached 30.0%–76.5% and 55.0%–88.2% after 32 and 46 days, respectively. View Full-Text
Keywords: biocontrol; entomopathogenic fungi; Hylobius abietis; Hypocreales; virulence biocontrol; entomopathogenic fungi; Hylobius abietis; Hypocreales; virulence
Show Figures

Figure 1

MDPI and ACS Style

Barta, M.; Lalík, M.; Rell, S.; Kunca, A.; Horáková, M.K.; Mudrončeková, S.; Galko, J. Hypocrealean Fungi Associated with Hylobius abietis in Slovakia, Their Virulence against Weevil Adults and Effect on Feeding Damage in Laboratory. Forests 2019, 10, 634.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop