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Open AccessArticle

Complete Chloroplast Genome of Pinus densiflora Siebold & Zucc. and Comparative Analysis with Five Pine Trees

1
National Institute of Forest Science, Department of Forest Bio-Resources, Suwon 16631, Korea
2
PHYZEN Genome Institute, 605, Baekgoong Plaza1, Seongnam-si, Gyeonggi-do 13558, Korea
3
Department of Plant Science, Plant Genomics and Breeding Institue, and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Forests 2019, 10(7), 600; https://doi.org/10.3390/f10070600
Received: 15 May 2019 / Revised: 11 July 2019 / Accepted: 16 July 2019 / Published: 19 July 2019
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Abstract

Pinus densiflora (Korean red pine) is widely distributed in East Asia and considered one of the most important species in Korea. In this study, the complete chloroplast genome of P. densiflora was sequenced by combining the advantages of Oxford Nanopore MinION and Illumina MiSeq. The sequenced genome was then compared with that of a previously published conifer plastome. The chloroplast genome was found to be circular and comprised of a quadripartite structure, including 113 genes encoding 73 proteins, 36 tRNAs and 4 rRNAs. It had short inverted repeat regions and lacked ndh gene family genes, which is consistent with other Pinaceae species. The gene content of P. densiflora was found to be most similar to that of P. sylvestris. The newly attempted sequencing method could be considered an alternative method for obtaining accurate genetic information, and the chloroplast genome sequence of P. densiflora revealed in this study can be used in the phylogenetic analysis of Pinus species. View Full-Text
Keywords: chloroplast; genome; pine tree; Oxford Nanopore Technology (ONT); MiSeq chloroplast; genome; pine tree; Oxford Nanopore Technology (ONT); MiSeq
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Kang, H.-I.; Lee, H.O.; Lee, I.H.; Kim, I.S.; Lee, S.-W.; Yang, T.J.; Shim, D. Complete Chloroplast Genome of Pinus densiflora Siebold & Zucc. and Comparative Analysis with Five Pine Trees. Forests 2019, 10, 600.

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