Next Article in Journal
The Characteristics of Soil C, N, and P Stoichiometric Ratios as Affected by Geological Background in a Karst Graben Area, Southwest China
Next Article in Special Issue
Cloning, Characterization and Expression of the Phenylalanine Ammonia-Lyase Gene (PaPAL) from Spruce Picea asperata
Previous Article in Journal
Comparison of Three Algorithms to Estimate Tree Stem Diameter from Terrestrial Laser Scanner Data
Previous Article in Special Issue
Complete Chloroplast Genome Sequence and Phylogenetic Analysis of Quercus bawanglingensis Huang, Li et Xing, a Vulnerable Oak Tree in China
Open AccessArticle

Complete Chloroplast Genome of Pinus densiflora Siebold & Zucc. and Comparative Analysis with Five Pine Trees

National Institute of Forest Science, Department of Forest Bio-Resources, Suwon 16631, Korea
PHYZEN Genome Institute, 605, Baekgoong Plaza1, Seongnam-si, Gyeonggi-do 13558, Korea
Department of Plant Science, Plant Genomics and Breeding Institue, and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Forests 2019, 10(7), 600;
Received: 15 May 2019 / Revised: 11 July 2019 / Accepted: 16 July 2019 / Published: 19 July 2019
PDF [2965 KB, uploaded 19 July 2019]


Pinus densiflora (Korean red pine) is widely distributed in East Asia and considered one of the most important species in Korea. In this study, the complete chloroplast genome of P. densiflora was sequenced by combining the advantages of Oxford Nanopore MinION and Illumina MiSeq. The sequenced genome was then compared with that of a previously published conifer plastome. The chloroplast genome was found to be circular and comprised of a quadripartite structure, including 113 genes encoding 73 proteins, 36 tRNAs and 4 rRNAs. It had short inverted repeat regions and lacked ndh gene family genes, which is consistent with other Pinaceae species. The gene content of P. densiflora was found to be most similar to that of P. sylvestris. The newly attempted sequencing method could be considered an alternative method for obtaining accurate genetic information, and the chloroplast genome sequence of P. densiflora revealed in this study can be used in the phylogenetic analysis of Pinus species. View Full-Text
Keywords: chloroplast; genome; pine tree; Oxford Nanopore Technology (ONT); MiSeq chloroplast; genome; pine tree; Oxford Nanopore Technology (ONT); MiSeq

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Kang, H.-I.; Lee, H.O.; Lee, I.H.; Kim, I.S.; Lee, S.-W.; Yang, T.J.; Shim, D. Complete Chloroplast Genome of Pinus densiflora Siebold & Zucc. and Comparative Analysis with Five Pine Trees. Forests 2019, 10, 600.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Forests EISSN 1999-4907 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top