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Open AccessArticle

In Vitro Propagation of Oriental White Oak Quercus aliena Blume

by Qiansheng Li 1, Mengmeng Gu 1 and Min Deng 2,3,*
Department of Horticultural Sciences, Texas A&M AgriLife Extension Service, College Station, TX 77843, USA
Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai 201602, China
Southeast Asia Biodiversity Research Institute, Chinese Academy of Sciences, Menglun, Mengla 666303, China
Author to whom correspondence should be addressed.
Forests 2019, 10(6), 463;
Received: 28 April 2019 / Revised: 16 May 2019 / Accepted: 23 May 2019 / Published: 28 May 2019
(This article belongs to the Section Forest Ecophysiology and Biology)
Quercus aliena Blume, also known as the oriental white oak, is a widespread species in temperate forests of East Asia with significant ecological and economical importance. Establishing an efficient vegetative propagation system is important for its germplasm conservation and breeding program. Protocols of micropropagation from shoot tips and nodal segments were investigated in order to produce uniform high-quality seedlings. Nodal segments from 18 month old seedlings were used as explants to initiate the aseptic culture. The highest bud proliferation was achieved by subculturing the explants on 1/2 strength woody plant medium (WPM) with 2.0 mg·L−1 BA. WPM with 0.5 mg·L−1 BA and 0.05 mg·L−1 IBA was the best medium for subculture to obtain the vigorous regenerated shoots in this experiment. Nodal segments without shoot tips had a higher adventitious bud proliferation rate than those with shoot tips. The highest rate (41.5%) of rooting in vitro was induced by using WPM with 1.0 mg·L−1 IBA and 5 g·L−1 activated charcoal. Ex vitro rooting by dipping the proliferated shoots with 500 mg·L−1 IBA solution, then transplanting directly to potting mix with 50% peat and 50% horticultural perlite fostered the highest rooting percentage and survival rate of the plantlets. View Full-Text
Keywords: ex vitro rooting; micropropagation; Quercus aliena; tissue culture; vegetative propagation ex vitro rooting; micropropagation; Quercus aliena; tissue culture; vegetative propagation
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Li, Q.; Gu, M.; Deng, M. In Vitro Propagation of Oriental White Oak Quercus aliena Blume. Forests 2019, 10, 463.

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