Genome-Wide Identification and Characterization of Heat-Shock Transcription Factors in Rubber Tree
by
Yan Li 1,2,3
, Wencai Yu 4, Yueyi Chen 2,3, Shuguang Yang 2,3, Shaohua Wu 2,3, Jinquan Chao 2,3, Xinlong Wang 2,3 and Wei-Min Tian 2,3,*
Yan Li 1,2,3, Wencai Yu 4, Yueyi Chen 2,3, Shuguang Yang 2,3, Shaohua Wu 2,3, Jinquan Chao 2,3, Xinlong Wang 2,3 and Wei-Min Tian 2,3,*
1
College of Tropical Crops, Hainan University, Haikou 570228, China
2
Ministry of Agriculture Key Laboratory of Biology and Genetic Resources of Rubber Tree, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
3
State Key Laboratory Breeding Base of Cultivation and Physiology for Tropical Crops, Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China
4
Yunnan Institute of Tropical Crops, Jinghong 666100, China
*
Author to whom correspondence should be addressed.
Forests 2019, 10(12), 1157; https://doi.org/10.3390/f10121157
Received: 2 November 2019 / Revised: 2 December 2019 / Accepted: 10 December 2019 / Published: 17 December 2019
(This article belongs to the Section Forest Ecophysiology and Biology)
Heat-shock transcription factors (Hsfs) play a pivotal role in the response of plants to various stresses. The present study aimed to characterize the Hsf genes in the rubber tree, a primary global source of natural rubber. In this study, 30 Hsf genes were identified in the rubber tree using genome-wide analysis. They possessed a structurally conserved DNA-binding domain and an oligomerization domain. On the basis of the length of the insert region between HR-A and HR-B in the oligomerization domain, the 30 members were clustered into three classes, Classes A (18), B (10), and C (2). Members within the same class shared highly conserved gene structures and protein motifs. The background expression levels of 11 genes in cold-tolerant rubber-tree clone 93-14 were significantly higher than those in cold-sensitive rubber-tree clone Reken501, while four genes exhibited inverse expression patterns. Upon cold stress, 20 genes were significantly upregulated in 93-114. Of the upregulated genes, HbHsfA2b, HbHsfA3a, and HbHsfA7a were also significantly upregulated in three other cold-tolerant rubber-tree clones at one or more time intervals upon cold stress. Their nuclear localization was verified, and the protein–protein interaction network was predicted. This study provides a basis for dissecting Hsf function in the enhanced cold tolerance of the rubber tree.
View Full-Text
Keywords:
Hevea brasiliensis Muell. Arg.; heat-shock transcription factor; cold stress; cold-sensitive rubber-tree clones; cold-tolerant rubber-tree clones
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
- Supplementary File 1:
ZIP-Document (ZIP, 628 KB)
MDPI and ACS Style
Li, Y.; Yu, W.; Chen, Y.; Yang, S.; Wu, S.; Chao, J.; Wang, X.; Tian, W.-M. Genome-Wide Identification and Characterization of Heat-Shock Transcription Factors in Rubber Tree. Forests 2019, 10, 1157.
Show more citation formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
