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Electrochemical Hydrogel Lithography of Calcium-Alginate Hydrogels for Cell Culture

1
Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan
2
WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8579, Japan
*
Authors to whom correspondence should be addressed.
Academic Editors: Sandra Van Vlierberghe and Peter Dubruel
Materials 2016, 9(9), 744; https://doi.org/10.3390/ma9090744
Received: 31 July 2016 / Revised: 12 August 2016 / Accepted: 22 August 2016 / Published: 31 August 2016
(This article belongs to the Special Issue Smart Hydrogels for (Bio)printing Applications)
Here we propose a novel electrochemical lithography methodology for fabricating calcium-alginate hydrogels having controlled shapes. We separated the chambers for Ca2+ production and gel formation with alginate with a semipermeable membrane. Ca2+ formed in the production chamber permeated through the membrane to fabricate a gel structure on the membrane in the gel formation chamber. When the calcium-alginate hydrogels were modified with collagen, HepG2 cells proliferated on the hydrogels. These results show that electrochemical hydrogel lithography is useful for cell culture. View Full-Text
Keywords: electrodeposition; electrochemical hydrogel lithography; calcium-alginate hydrogel; cell culture electrodeposition; electrochemical hydrogel lithography; calcium-alginate hydrogel; cell culture
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MDPI and ACS Style

Ozawa, F.; Ino, K.; Shiku, H.; Matsue, T. Electrochemical Hydrogel Lithography of Calcium-Alginate Hydrogels for Cell Culture. Materials 2016, 9, 744.

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