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Materials 2016, 9(11), 889;

A Natural Bacterium-Produced Membrane-Bound Nanocarrier for Drug Combination Therapy

College of Chemical Engineering, Huaqiao University, Xiamen 361021, China
Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen 361021, China
Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen 361021, China
Author to whom correspondence should be addressed.
Academic Editor: Jung Ho Je
Received: 17 September 2016 / Revised: 30 October 2016 / Accepted: 31 October 2016 / Published: 2 November 2016
(This article belongs to the Section Structure Analysis and Characterization)
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To minimize the non-specific toxicity of drug combination during cancer therapy, we prepared a new system synthesized from bacteria to deliver the anticancer drugs cytosine arabinoside (Ara-C) and daunorubicin (DNR). In this study, we selected genipin (GP) and poly-l-glutamic acid (PLGA) as dual crosslinkers. Herewith, we demonstrated the preparation, characterization and in vitro antitumor effects of Ara-C and DNR loaded GP-PLGA-modified bacterial magnetosomes (BMs) (ADBMs-P). The results show that this new system is stable and exhibits optimal drug-loading properties. The average diameters of BMs and ADBMs-P were 42.0 ± 8.6 nm and 65.5 ± 8.9 nm, respectively, and the zeta potential of ADBMs-P (−42.0 ± 6.4 mV) was significantly less than that of BMs (−28.6 ± 7.6 mV). The optimal encapsulation efficiency and drug loading of Ara-C were 68.4% ± 9.4% and 32.4% ± 2.9%, respectively, and those of DNR were 36.1% ± 2.5% and 17.9% ± 1.6%. Interestingly, this system also exhibits long-term release behaviour sequentially, without an initial burst release. The Ara-C drug continued to release about 85% within 40 days, while DNR release lasted only for 13 days. Moreover, similar to free drugs, ADBMs-Ps are strongly cytotoxic to cancer cells in vitro (HL-60 cells), with the inhibition rate approximately 96%. This study reveals that this new system has a potential for drug delivery application in the future, especially for combination therapy. View Full-Text
Keywords: magnetosomes; natural carrier; drug combination therapy; dual crosslinkers magnetosomes; natural carrier; drug combination therapy; dual crosslinkers

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Long, R.; Liu, Y.; Dai, Q.; Wang, S.; Deng, Q.; Zhou, X. A Natural Bacterium-Produced Membrane-Bound Nanocarrier for Drug Combination Therapy. Materials 2016, 9, 889.

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