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Open AccessArticle

Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications

by Bae Hoon Lee 1,*,†, Nathaniel Lum 1,†, Li Yuan Seow 1,†, Pei Qi Lim 1 and Lay Poh Tan 1,2,*
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore
Singapore Centre for 3D Printing (SC3DP), Singapore 639798, Singapore
Authors to whom correspondence should be addressed.
Academic Editors: Nicole Zander, Chee Kai Chua, Wai Yee Yeong and Jia An
Materials 2016, 9(10), 797;
Received: 7 August 2016 / Revised: 14 September 2016 / Accepted: 20 September 2016 / Published: 24 September 2016
(This article belongs to the Special Issue 3D Printing for Biomedical Engineering)
Gelatin methacryloyl (GelMA) has been increasingly considered as an important bioink material due to its tailorable mechanical properties, good biocompatibility, and ability to be photopolymerized in situ as well as printability. GelMA can be classified into two types: type A GelMA (a product from acid treatment) and type B GelMA (a product from alkali treatment). In current literature, there is little research on the comparison of type A GelMA and type B GelMA in terms of synthesis, rheological properties, and printability for bioink applications. Here, we report the synthesis, rheological properties, and printability of types A and B GelMA. Types A and B GelMA samples with different degrees of substitution (DS) were prepared in a controllable manner by a time-lapse loading method of methacrylic anhydride (MAA) and different feed ratios of MAA to gelatin. Type B GelMA tended to have a slightly higher DS compared to type A GelMA, especially in a lower feed ratio of MAA to gelatin. All the type A and type B GelMA solutions with different DS exhibited shear thinning behaviours at 37 °C. However, only GelMA with a high DS had an easy-to-extrude feature at room temperature. The cell-laden printed constructs of types A and B GelMA at 20% w/v showed around 75% cell viability. View Full-Text
Keywords: gelatin; methacryloyl; bioink; type A and type B; 3D bioprinting gelatin; methacryloyl; bioink; type A and type B; 3D bioprinting
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Lee, B.H.; Lum, N.; Seow, L.Y.; Lim, P.Q.; Tan, L.P. Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications. Materials 2016, 9, 797.

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