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Liquefied Wood as Inexpensive Precursor-Feedstock for Bio-Mediated Incorporation of (R)-3-Hydroxyvalerate into Polyhydroxyalkanoates

Institute of Chemistry, University of Graz, NAWI Graz, Heinrichstrasse 28/III, Graz 8010, Austria
ARENA—Association for resource efficient and sustainable technologies, Inffeldgasse 23, Graz 8010, Austria
Department of Genetics, Institut de la Vision, 17 rue Moreau, 75012 Paris, France
Departament d’Òptica i Optometria, Universitat Politècnica de Catalunya Barcelona Tech, Sant Nebridi 22, 08222 Terrassa, Barcelona, Spain
Laboratory for Polymer Chemistry and Technology, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia
Author to whom correspondence should be addressed.
Academic Editor: Marek M. Kowalczuk
Materials 2015, 8(9), 6543-6557;
Received: 19 August 2015 / Accepted: 16 September 2015 / Published: 23 September 2015
(This article belongs to the Special Issue Biodegradable and Bio-Based Polymers)
Liquefied wood (LW) prepared in a microwave process was applied as a novel; inexpensive precursor feedstock for incorporation of (R)-3-hydroxyvalerate (3HV) into polyhydroxyalkanoate (PHA) biopolyesters in order to improve the biopolyester’s material quality; Cupriavidus necator was applied as microbial production strain. For proof of concept, pre-experiments were carried out on a shake flask scale using different mixtures of glucose and LW as carbon source. The results indicate that LW definitely acts as a 3HV precursor, but, at the same time, displays toxic effects on C. necator at concentrations exceeding 10 g/L. Based on these findings, PHA biosynthesis under controlled conditions was performed using a fed-batch feeding regime on a bioreactor scale. As major outcome, a poly(3HB-co-0.8%-3HV) copolyester was obtained displaying a desired high molar mass of Mw = 5.39 × 105 g/mol at low molar-mass dispersity (ĐM of 1.53), a degree of crystallinity (Xc) of 62.1%, and melting temperature Tm (176.3 °C) slightly lower than values reported for poly([R]-3-hydroxybutyrate) (PHB) homopolyester produced by C. necator; thus, the produced biopolyester is expected to be more suitable for polymer processing purposes. View Full-Text
Keywords: biopolymers; copolyester; (R)-3-Hydroxyvalerate (3HV); liquefied wood; polyhydroxyalkanoates (PHA); precursor substrates biopolymers; copolyester; (R)-3-Hydroxyvalerate (3HV); liquefied wood; polyhydroxyalkanoates (PHA); precursor substrates
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MDPI and ACS Style

Koller, M.; Dias, M.M.S.; Rodríguez-Contreras, A.; Kunaver, M.; Žagar, E.; Kržan, A.; Braunegg, G. Liquefied Wood as Inexpensive Precursor-Feedstock for Bio-Mediated Incorporation of (R)-3-Hydroxyvalerate into Polyhydroxyalkanoates. Materials 2015, 8, 6543-6557.

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