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Liquefied Wood as Inexpensive Precursor-Feedstock for Bio-Mediated Incorporation of (R)-3-Hydroxyvalerate into Polyhydroxyalkanoates

1
Institute of Chemistry, University of Graz, NAWI Graz, Heinrichstrasse 28/III, Graz 8010, Austria
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ARENA—Association for resource efficient and sustainable technologies, Inffeldgasse 23, Graz 8010, Austria
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Department of Genetics, Institut de la Vision, 17 rue Moreau, 75012 Paris, France
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Departament d’Òptica i Optometria, Universitat Politècnica de Catalunya Barcelona Tech, Sant Nebridi 22, 08222 Terrassa, Barcelona, Spain
5
Laboratory for Polymer Chemistry and Technology, National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia
*
Author to whom correspondence should be addressed.
Academic Editor: Marek M. Kowalczuk
Materials 2015, 8(9), 6543-6557; https://doi.org/10.3390/ma8095321
Received: 19 August 2015 / Accepted: 16 September 2015 / Published: 23 September 2015
(This article belongs to the Special Issue Biodegradable and Bio-Based Polymers)
Liquefied wood (LW) prepared in a microwave process was applied as a novel; inexpensive precursor feedstock for incorporation of (R)-3-hydroxyvalerate (3HV) into polyhydroxyalkanoate (PHA) biopolyesters in order to improve the biopolyester’s material quality; Cupriavidus necator was applied as microbial production strain. For proof of concept, pre-experiments were carried out on a shake flask scale using different mixtures of glucose and LW as carbon source. The results indicate that LW definitely acts as a 3HV precursor, but, at the same time, displays toxic effects on C. necator at concentrations exceeding 10 g/L. Based on these findings, PHA biosynthesis under controlled conditions was performed using a fed-batch feeding regime on a bioreactor scale. As major outcome, a poly(3HB-co-0.8%-3HV) copolyester was obtained displaying a desired high molar mass of Mw = 5.39 × 105 g/mol at low molar-mass dispersity (ĐM of 1.53), a degree of crystallinity (Xc) of 62.1%, and melting temperature Tm (176.3 °C) slightly lower than values reported for poly([R]-3-hydroxybutyrate) (PHB) homopolyester produced by C. necator; thus, the produced biopolyester is expected to be more suitable for polymer processing purposes. View Full-Text
Keywords: biopolymers; copolyester; (R)-3-Hydroxyvalerate (3HV); liquefied wood; polyhydroxyalkanoates (PHA); precursor substrates biopolymers; copolyester; (R)-3-Hydroxyvalerate (3HV); liquefied wood; polyhydroxyalkanoates (PHA); precursor substrates
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MDPI and ACS Style

Koller, M.; Dias, M.M.S.; Rodríguez-Contreras, A.; Kunaver, M.; Žagar, E.; Kržan, A.; Braunegg, G. Liquefied Wood as Inexpensive Precursor-Feedstock for Bio-Mediated Incorporation of (R)-3-Hydroxyvalerate into Polyhydroxyalkanoates. Materials 2015, 8, 6543-6557.

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