Next Article in Journal
Optothermal Switching of Cholesteric Liquid Crystals: A Study of Azobenzene Derivatives and Laser Wavelengths
Previous Article in Journal
Spark Plasma Sintering of Commercial Zirconium Carbide Powders: Densification Behavior and Mechanical Properties
Open AccessArticle

In Vitro Studies of Polyhedral Oligo Silsesquioxanes: Evidence for Their Low Cytotoxicity

1
Department of General Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, Lodz 90-236, Poland
2
Centre of Molecular and Macromolecular Sciences, Sienkiewicza 112, Lodz 90-236, Poland
3
Leibniz-Institut für Polymerforschung Dresden e.V., Hohe Strasse 6, Dresden 01069, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Aldo Boccaccini
Materials 2015, 8(9), 6062-6070; https://doi.org/10.3390/ma8095291
Received: 26 June 2015 / Revised: 25 August 2015 / Accepted: 6 September 2015 / Published: 10 September 2015
(This article belongs to the Section Biomaterials)
As scientific literature considers polyhedral oligosilsesquioxanes (POSS) as potential drug delivery systems, it is necessary to check their impact on mammalian cells. Toxicity of octaammonium chloride salt of octaaminopropyl polyhedral oligomeric silsesquioxane (oap-POSS) towards two cell lines: mouse neuroblastoma (N2a) and embryonic mouse hippocampal cells (mHippoE-18) was studied. Experiments consisted of analysis of a cell cycle, cell viability, amount of apoptotic and necrotic cells, and generation of reactive oxygen species (ROS). POSS caused a shift in the cell population from the S and M/G2 phases to the G0/G1 phase. However, the changes affected less than 10% of the cell population and were not accompanied by increased cytotoxicity. POSS did not induce either apoptosis or necrosis and did not generate reactive oxygen species. A cytotoxicity profile of POSS makes it a promising starting material as drug carrier. View Full-Text
Keywords: POSS; silsesquioxane; in vitro toxicity; MTT assay; cell cycle; apoptosis; necrosis; reactive oxygen species POSS; silsesquioxane; in vitro toxicity; MTT assay; cell cycle; apoptosis; necrosis; reactive oxygen species
Show Figures

Figure 1

MDPI and ACS Style

Janaszewska, A.; Gradzinska, K.; Marcinkowska, M.; Klajnert-Maculewicz, B.; Stanczyk, W.A. In Vitro Studies of Polyhedral Oligo Silsesquioxanes: Evidence for Their Low Cytotoxicity. Materials 2015, 8, 6062-6070.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop