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Materials 2015, 8(9), 6004-6017;

Constructing Biopolymer-Inorganic Nanocomposite through a Biomimetic Mineralization Process for Enzyme Immobilization

1,* , 1
School of Material Science and Chemical Engineering, Tianjin University of Science and Technology, Tianjin 300457, China
Tianjin Synthetic Material Research Institute, Tianjin 300220, China
Key Laboratory for Green Chemical Technology of Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editor: Armando J. D. Silvestre
Received: 19 May 2015 / Accepted: 15 July 2015 / Published: 9 September 2015
(This article belongs to the Special Issue Biobased Nanocomposite Functional Materials)
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Inspired by biosilicification, biomimetic polymer-silica nanocomposite has aroused a lot of interest from the viewpoints of both scientific research and technological applications. In this study, a novel dual functional polymer, NH2-Alginate, is synthesized through an oxidation-amination-reduction process. The “catalysis function” ensures the as-prepared NH2-Alginate inducing biomimetic mineralization of silica from low concentration precursor (Na2SiO3), and the “template function” cause microscopic phase separation in aqueous solution. The diameter of resultant NH2-Alginate micelles in aqueous solution distributed from 100 nm to 1.5 μm, and is influenced by the synthetic process of NH2-Alginate. The size and morphology of obtained NH2-Alginate/silica nanocomposite are correlated with the micelles. NH2-Alginate/silica nanocomposite was subsequently utilized to immobilize β-Glucuronidase (GUS). The harsh condition tolerance and long-term storage stability of the immobilized GUS are notably improved due to the buffering effect of NH2-Alginate and cage effect of silica matrix. View Full-Text
Keywords: biomimetic mineralization; nanocomposite; enzyme immobilization; silica; alginate; template biomimetic mineralization; nanocomposite; enzyme immobilization; silica; alginate; template

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Li, J.; Ma, J.; Jiang, T.; Wang, Y.; Wen, X.; Li, G. Constructing Biopolymer-Inorganic Nanocomposite through a Biomimetic Mineralization Process for Enzyme Immobilization. Materials 2015, 8, 6004-6017.

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