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Isolation and Characterization of Nano-Hydroxyapatite from Salmon Fish Bone

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Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Korea
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Department of Marine-bio Convergence Science, Pukyong National University, Busan 608-737, Korea
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Stem Cell Unit, Department of Anatomy, King Saud University, Riyadh 12372, Saudi Arabia
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Dental Biomaterials Research Chair, King Saud University, Riyadh 12372, Saudi Arabia
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Specialized Graduate School Science & Technology Convergence, Pukyong National University, Busan 608-739, Korea
*
Authors to whom correspondence should be addressed.
Academic Editor: Nicola Pugno
Materials 2015, 8(8), 5426-5439; https://doi.org/10.3390/ma8085253
Received: 5 June 2015 / Revised: 6 August 2015 / Accepted: 7 August 2015 / Published: 21 August 2015
Nano-Hydroxyapatite (nHA) was isolated from salmon bone by alkaline hydrolysis. The resulting nHA was characterized using several analytical tools, including thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and transmission electron microscopy (TEM), to determine the purity of the nHA sample. The removal of organic matter from the raw fish was confirmed by TGA. FT-IR confirmed the presence of a carbonated group and the similarities to synthetic Sigma HA. XRD revealed that the isolated nHA was amorphous. Microscopy demonstrated that the isolated nHA possessed a nanostructure with a size range of 6–37 nm. The obtained nHA interacted with mesenchymal stem cells (MSCs) and was non-toxic. Increased mineralization was observed for nHA treated MSCs compared to the control group. These results suggest that nHA derived from salmon is a promising biomaterial in the field of bone tissue engineering. View Full-Text
Keywords: hydroxyapatite; salmon; fish; alkaline hydrolysis hydroxyapatite; salmon; fish; alkaline hydrolysis
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Venkatesan, J.; Lowe, B.; Manivasagan, P.; Kang, K.-H.; Chalisserry, E.P.; Anil, S.; Kim, D.G.; Kim, S.-K. Isolation and Characterization of Nano-Hydroxyapatite from Salmon Fish Bone. Materials 2015, 8, 5426-5439.

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