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Fast Blue RR—Siloxane Derivatized Materials Indicate Wound Infection Due to a Deep Blue Color Development

1
ACIB—Austrian Centre of Industrial Biotechnology, Konrad-Lorenz Strasse 203630 Tuln, Graz 8010, Austria
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Department of Environmental Biotechnology, University of Natural Resources and Life Sciences Vienna, Konrad Lorenz Strasse 20, 3430 Tulln an der Donau, Vienna 1180, Austria
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Department of Organic Chemistry, Technical University Graz, Stremayrgasse 16, 8010 Graz, Austria
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Functional Surfaces & Nanostructures, PROFACTOR GmbH, Im Stadtgut A2, Steyr-Gleink 4407, Austria
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Qualizyme GmbH, Neue Stiftingtalstrasse 2, Graz 8010, Austria
*
Author to whom correspondence should be addressed.
Academic Editor: Jun-ichi Anzai
Materials 2015, 8(10), 6633-6639; https://doi.org/10.3390/ma8105329
Received: 29 June 2015 / Revised: 10 September 2015 / Accepted: 21 September 2015 / Published: 25 September 2015
There is a strong need for simple and fast methods for wound infection determination. Myeloperoxidase, an immune system-derived enzyme was found to be a suitable biomarker for wound infection. Hence, alkoxysilane-derivatized Fast Blue RR was immobilized via simple hydrolytic polymerization. The resulting enzyme-responsive siloxane layers were incubated with myeloperoxidase, wound fluid or hemoglobin. The reaction was monitored via HPLC measurements and the color development quantified spectrophotometrically. Myeloperoxidase was indeed able to oxidize immobilized Fast Blue RR leading to a blue colored product. No conversion was detected in non-infected wound fluids. The visible color changes of these novel materials towards blue enable an easy distinction between infected and non-infected wound fluids. View Full-Text
Keywords: immobilization; Fast Blue RR; alkoxysilane derivatized Fast Blue RR; myeloperoxidase; infection detection immobilization; Fast Blue RR; alkoxysilane derivatized Fast Blue RR; myeloperoxidase; infection detection
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Schiffer, D.; Tegl, G.; Vielnascher, R.; Weber, H.; Schoeftner, R.; Wiesbauer, H.; Sigl, E.; Heinzle, A.; Guebitz, G.M. Fast Blue RR—Siloxane Derivatized Materials Indicate Wound Infection Due to a Deep Blue Color Development. Materials 2015, 8, 6633-6639.

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