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Materials 2014, 7(2), 1046-1054;

Vascular Endothelial Growth Factor (VEGF) Detection Using an Aptamer and PNA-Based Bound/Free Separation System

Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 13 December 2013 / Revised: 15 January 2014 / Accepted: 28 January 2014 / Published: 11 February 2014
(This article belongs to the Special Issue Functional Materials and Proteins for Bio-Sensing Applications)
Full-Text   |   PDF [330 KB, uploaded 11 February 2014]


We have developed a bound/free separation system using a vascular endothelial growth factor (VEGF) aptamer and a peptide nucleic acid (PNA) to detect VEGF. In this system, we designed capture PNA (CaPNA), which hybridizes with the aptamer in the absence of the target protein, but does not hybridize with the aptamer in the presence of the target protein due to steric hindrance and/or stabilization of the aptamer’s structure. By removing the aptamers not bound to the target protein using CaPNA immobilized beads, we can detect the target protein by measuring signals labeled with the aptamer in the supernatant. In this study, we detected VEGF using CaPNA-immobilized beads without the time-consuming washing step. This simple and rapid system can detect 25 nM of VEGF in 15 min. View Full-Text
Keywords: aptamer; bound free separation; peptide nucleic acid aptamer; bound free separation; peptide nucleic acid
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Mita, C.; Abe, K.; Fukaya, T.; Ikebukuro, K. Vascular Endothelial Growth Factor (VEGF) Detection Using an Aptamer and PNA-Based Bound/Free Separation System. Materials 2014, 7, 1046-1054.

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