The Effect of Synovial Fluid Enzymes on the Biodegradability of Collagen and Fibrin Clots
Abstract
:1. Introduction
Enzyme | Concentration in the knee | Experimental Concentration |
---|---|---|
MMP-1 | 177 to 279 µg/mL (Gysen, 1985) [18] | 250 µg/mL |
Elastase | 0.041 to 101.8 µg/mL (Kleesiek, 1986) [19] | 100 µg/mL |
Plasmin | 23 to 55 µg/mL (Kummer, 1992) [20] | 40 µg/mL |
2. Results and Discussion
2.1. MMP-1 Degradation
2.2. Elastase Degradation
2.3. PBS Degradation
2.4. Plasmin Degradation
2.5. Water Degradation
2.6. Cell Proliferation
2.7. Scaffold Contraction
3. Experimental Section
3.1. Manufacturing of Atelocollagen Scaffolds
3.2. Gelatin Scaffolds
3.3. Preparation of Fibrin Clot Scaffolds
3.4. Preparation of Blood Clot Scaffolds
3.5. Preparation of Enzyme Stock Solutions
3.6. Enzyme Degradation Experiment
3.7. Cell Source
3.8. Measurement of Cell Proliferation in Scaffolds
3.9. Statistical Analysis
4. Discussion
5. Conclusions
Acknowledgment
References
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Palmer, M.; Stanford, E.; Murray, M.M. The Effect of Synovial Fluid Enzymes on the Biodegradability of Collagen and Fibrin Clots. Materials 2011, 4, 1469-1482. https://doi.org/10.3390/ma4081469
Palmer M, Stanford E, Murray MM. The Effect of Synovial Fluid Enzymes on the Biodegradability of Collagen and Fibrin Clots. Materials. 2011; 4(8):1469-1482. https://doi.org/10.3390/ma4081469
Chicago/Turabian StylePalmer, Matthew, Elizabeth Stanford, and Martha M. Murray. 2011. "The Effect of Synovial Fluid Enzymes on the Biodegradability of Collagen and Fibrin Clots" Materials 4, no. 8: 1469-1482. https://doi.org/10.3390/ma4081469